Figure 1. Conditional deletion of pfn1 in HSCs. (A) Schematic of pfn1 floxed allele showing deletion of floxed exon 1 following Cre recombinase activity. Use of Scl-Cre-ERT results in specific deletion of the pfn1 gene in HSCs following tamoxifen treatment. (B) Genotype analyses of LT-HSCs (Lin−Sca-1+Kit+Flk2−CD34−, LSKFC) and Lin+, Lin−, Lin−Kit+ (LK) cells and whole BM (WBM) cells at 1 and 6 days after tamoxifen injections. Deletion of pfn1 is indicated by the arrow (700 bp).
Figure 1

Conditional deletion of pfn1 in HSCs. (A) Schematic of pfn1 floxed allele showing deletion of floxed exon 1 following Cre recombinase activity. Use of Scl-Cre-ERT results in specific deletion of the pfn1 gene in HSCs following tamoxifen treatment. (B) Genotype analyses of LT-HSCs (Lin−Sca-1+Kit+Flk2−CD34−, LSKFC) and Lin+, Lin−, Lin−Kit+ (LK) cells and whole BM (WBM) cells at 1 and 6 days after tamoxifen injections. Deletion of pfn1 is indicated by the arrow (700 bp).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal