Figure 1. Typical colonies observed in ET and in RARS with ET features. Colonies are shown after May-Grünwald-Giemsa staining of collagen gels and are typical of ET (A-C) or of RARS with ET features (D-E). (A) Endogenous megakaryocytic colony, grown without serum or cytokine. (B,D) Erythroid colonies grown in the presence of cytokines; note the small size of the RARS colony (D). (C) Detail of erythroblasts of the colony shown in panel B. (E) Detail of erythroblasts of the colony shown in panel D; note extensive cell death. Images were obtained using a Leica microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 10×/0.80 numeric aperture (NA) (A,B,D) or a 100×/0.80 NA (C,E) objective. Images were acquired using a Sony Power HAD DXC-950P camera (Sony, Tokyo, Japan) and Tribun ICS/Thunder 17 acquisition software. Images were processed using Microsoft PowerPoint 2000 (Microsoft, Redmond, WA).
Figure 1.

Typical colonies observed in ET and in RARS with ET features. Colonies are shown after May-Grünwald-Giemsa staining of collagen gels and are typical of ET (A-C) or of RARS with ET features (D-E). (A) Endogenous megakaryocytic colony, grown without serum or cytokine. (B,D) Erythroid colonies grown in the presence of cytokines; note the small size of the RARS colony (D). (C) Detail of erythroblasts of the colony shown in panel B. (E) Detail of erythroblasts of the colony shown in panel D; note extensive cell death. Images were obtained using a Leica microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 10×/0.80 numeric aperture (NA) (A,B,D) or a 100×/0.80 NA (C,E) objective. Images were acquired using a Sony Power HAD DXC-950P camera (Sony, Tokyo, Japan) and Tribun ICS/Thunder 17 acquisition software. Images were processed using Microsoft PowerPoint 2000 (Microsoft, Redmond, WA).

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