Figure 4. Immuno-FISH analysis of VEGF-treated muscles of mice that received a transplant at 15 days, 30 days, and 3 months after vector injection. Immunofluorescence staining for endothelial (CD31; A) and smooth muscle (α-SMA; B) antigens was combined with Y-chromosome fluorescent in situ hybridization. The vast majority of cells expressing either of the 2 markers were not found to contain a Y chromosome (enlargements in panels A and B). Very rare cells (< 1% of total Y-chromosome–positive nuclei) were positive for CD31 and Y-chromosome markers (shown in the enlargements in panel C); cells of donor origin and positive for the α-SMA antigen were never found in the tunica media of vessels. Blue indicates nuclei stained with DAPI; red, (A) CD31- and (B) α-SMA–positive cells; and yellow, Y chromosome.
Figure 4.

Immuno-FISH analysis of VEGF-treated muscles of mice that received a transplant at 15 days, 30 days, and 3 months after vector injection. Immunofluorescence staining for endothelial (CD31; A) and smooth muscle (α-SMA; B) antigens was combined with Y-chromosome fluorescent in situ hybridization. The vast majority of cells expressing either of the 2 markers were not found to contain a Y chromosome (enlargements in panels A and B). Very rare cells (< 1% of total Y-chromosome–positive nuclei) were positive for CD31 and Y-chromosome markers (shown in the enlargements in panel C); cells of donor origin and positive for the α-SMA antigen were never found in the tunica media of vessels. Blue indicates nuclei stained with DAPI; red, (A) CD31- and (B) α-SMA–positive cells; and yellow, Y chromosome.

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