GILZ involvement in TGFβ-induced alterations of DC functions. (A) MoDCs were treated on day 5 with DEX, TGFβ, or medium, and with control (siC) or GILZ (siGILZ) siRNA. On day 7, DC phenotype was determined. (B) MoDCs were treated on day 5 with control (siC) or GILZ (siGILZ) siRNA and with DEX, TGFβ, or medium. They were loaded with PPD, washed, and mixed with autologous CD4⁺ T lymphocytes on day 7. Lymphocyte proliferation was determined by Pkh26 fluorescence intensity. One typical experiment of 2 is shown.