Ex vivo transcriptome analysis of bispecific CAR-iNKT cells. (A) Immunophenotypic profiling of preinfusion CAR-iNKT cells and design of the experiment. (B) UMAP embedding of gene expression data of the preinfusion CAR-iNKT cells, and (C) bubble plot showing comparative expression levels of genes of immunological relevance in clusters 0, 1, and 2 from panel B. (D) UMAP embedding of gene expression data of all experimental groups after batch correction colored by cluster ID (top) and by sample (bottom). (E) Volcano plot showing differential gene expression between clusters 0 and 1 (log₂FC > 1 and Padj < .05). (F) Bubble plot showing comparative expression levels of genes of immunological relevance in clusters 0, 1, and 2. (G) Frequency of cells in different clusters over the time course of the experiment. (H) Dynamic variation in the score of indicated enriched pathways between the preinfusion product, and days 3 and 15 CAR-iNKT cells. All pathways are significantly different between samples (P < .001). (I) Dynamic variation in the proportion of KLRK1+/− cells between the preinfusion product, and days 3 and 15 CAR-iNKT cells. UMAP, Uniform Manifold Approximation and Projection.