AR expression in AML cells affects survival. (A) Western blot showing the expression of ARs and ERαs in purified 1° wild-type (WT) male and female AML cells. (B) Expression of Ar as assessed by quantitative polymerase chain reaction (qPCR) in WT male and female AML cells. Data were normalized to WT male AML cells and Gapdh housekeeping gene (n = 8-10 biologic replicates). (C) Western blot showing the expression of ARs in WT male and female AML cells. Each lane represents 1 biologic replicate. (D) Expression of Esr1 and Esr2 assessed by qPCR in WT male and female AML cells. The data were normalized to WT male AML cells and Gapdh housekeeping gene (n = 3-10 biologic replicates). (E) Western blot showing the expression of ERα in WT male and female AML cells. (F) Survival curve of WT female and male recipient mice secondarily transplanted with WT high and low AR–expressing AML donor cells (n = 10-11 in each group); Log-rank tests were used to indicate significance with ∗P < .05 and ∗∗P < .01. (G) Representative image of spleens isolated from recipients in panel F at the end point. (H) Spleen weights (mg) of recipient mice in panel F at the end point. Panels B,D,H, error bars represent the mean ± standard error of the mean (SEM) of replicates. ∗P < .05; ∗∗P < .01, as determined by 1-tailed unpaired Student t test.