In vitro–exhausted Vγ9Vδ2 T cells show an altered transcriptome. (A) Volcano plot representing overexpressed (red) and underexpressed (blue) genes, identified as the threshold of log twofold change >1 and P value <.05, comparing untreated to SKOV3/Zol-treated Vγ9Vδ2 T cells. (B) Heatmaps representing the selected -Log 10 (P > 10) genes expression, grouped by hand by major pathway for untreated and SKOV3/Zol-treated Vγ9Vδ2 T cells, untreated, SKOV3-treated and anti-CD3/CD28 -treated αβ T cells. (C-F) Diverse genes of interest expression, as a gene read count, between untreated (blue) and SKOV3/Zol-treated (red) Vγ9Vδ2 T cells. All statistical analyses were performed using unpaired t tests (∗P < .05; ∗∗P < .003; ∗∗∗P < .0005; ∗∗∗∗P < .0001, exact P values are written where a trend was observed).