Decreased effector functions and altered metabolic profile of in vitro–exhausted Vγ9Vδ2 T cells. (A) Frequency (%) of CD107a among treated (right) vs untreated (left) Vγ9Vδ2⁺ T cells after coculture with Zol-presensitized (triangle) or nonactivated (round) BLCL. Effector-to-target ratio ratio (E:T) 1:1. (B) Monitoring of mitochondrial OCR, picomoles per minute of treated (red) vs untreated (blue) Vγ9Vδ2 T cells after a mitostress experiment over time. (C) Basal respiration (picomoles per minute) measured as the mean of the 3 values in the absence of substrate. (D) ATP production (picomoles per minute) calculated as the difference between the maximum value after injection of oligomycin and the basal respiration. (E) Maximal respiration (picomoles per minute) measured as the maximum value after FCCP injection; n = 4 donors. All statistical analyses were performed using 2-way analysis of variance (ANOVA) (panel A) or unpaired t tests (panels C-E) (∗P < .05; ∗∗P < .003; ∗∗∗P < .0005; ∗∗∗∗P < .0001). BLCL, B-lymphoblastoid cell line.