Vγ9Vδ2 T cells treated with SKOV3/Zol show an exhausted phenotype. (A) Scheme of the 4 days coculture in vitro experiment between ex vivo expanded human Vγ9Vδ2 T cells and Zol-presensitized SKOV3 cells. (B) Analysis by flow cytometry of expression of viability marker (7AAD) on ex vivo–expanded human Vγ9Vδ2 T cells before (blue) and after (red) treatment; n> 4 donors. (C) Flow cytometry histogram and mean fluorescence intensity analysis of expression of PD-1, LAG-3, TIM-3 and TIGIT; n = 4 donors. (D) Dot plots from a representative donor of surface expression of PD-1, LAG-3, TIM-3 and TIGIT. (E) Calculated percentage of LAG-3 TIM-3 TIGIT triple positive human Vγ9Vδ2 T cells. (F) Dot plots from a representative donor of intracellular expression of TOX and c-Maf transcription factors. (G) Flow cytometry histogram and mean fluorescence intensity analysis of expression of TOX and c-Maf; n = 3 donors. All statistical analysis was performed using unpaired t tests (∗P < .05; ∗∗P < .003; ∗∗∗P < .0005; ∗∗∗∗P < .0001).