Importance of cDCs for the splenic retention and rapid clearance of Cd47^−/− RBCs transfused into WT recipient mice. (A) Representative flow cytometric plots (left panel) and the percentage (right panel) of CFSE-labeled Cd47^−/− RBCs among total splenic RBCs at 2 hours after IV injection of the CFSE-labeled cells in WT recipient mice previously subjected to a single injection of CLs (CL ×1) or PBS liposomes (PBS ×1) as in Figure 2A. The data in the right panel are means ± SEM for 3 recipient mice. (B) Representative flow cytometric plots (left panel) and the percentage (right panel) of CFSE-labeled Cd47^−/− RBCs among total splenic RBCs at 2 hours after IV injection of the CFSE-labeled cells in WT recipient mice previously subjected to 2 injections of CLs (CL ×2) or PBS liposomes (PBS ×2) as in Figure 2B. The data in the right panel are means ± SEM for 3 recipient mice. (C) Representative flow cytometric plots (left panel) and the percentage (right panel) of cDCs among total splenic cells of WT or CD11c^iDTRΔ mice at 2 days after DTx treatment. The data in the right panel are means ± SEM for 3 recipient mice. (D) Representative flow cytometric plots (left panel) and the percentage (right panel) of CFSE-positive Cd47^−/− RBCs among total splenic RBCs at 2 hours after IV cell injection in WT or CD11c^iDTRΔ mice that had been treated with DTx 2 days previously. The data in the right panel are means ± SEM for 3 recipient mice. (E) Clearance of transfused Cd47^−/− RBCs from peripheral blood of WT or CD11c^iDTRΔ mice at 2 days after DTx injection. Data are means ± SEM for 3 recipient mice. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001 by Student t test (A-D) or by 2-way repeated-measures ANOVA with Tukey’s multiple-comparison test (E). NS, not significant.