Figure 2.
Two injections of CLs abolish the rapid elimination of transfused Cd47−/− RBCs from WT recipient mice. (A) Representative flow cytometric plots of RPMs (upper left panel) and cDCs (lower left panel) and the percentage of RPMs among total splenic cells (upper right panel) and that of cDCs among total splenic cells (lower right panel) on day 7 after treatment of mice with 50 μL of CLs (CL ×1) or PBS liposomes (PBS ×1) on day 0. Data in the right panels are means ± SEM for 4 mice. (B) Representative flow cytometric plots of RPMs (upper left panel) and cDCs (lower left panel) and the percentage of RPMs among total splenic cells (upper right panel) and that of cDCs among total splenic cells (lower right panel) on day 2 after treatment of mice with 100 μL of CLs (CL ×2) or PBS liposomes (PBS ×2) on day 0 and day 1. Data in the right panels are means ± SEM for 3 mice. (C) Clearance of transfused Cd47−/− RBCs from peripheral blood of WT recipient mice treated with CLs (CL ×1) or PBS liposomes (PBS ×1) as in panel A. Data for WT recipient mice injected with WT RBCs but not treated with liposomes (WT RBCs [no treatment]) are also revealed. The data in the lower panel are a more detailed representation of those in the upper panel. All data are means ± SEM for 3 recipient mice. (D) Clearance of transfused Cd47−/− RBCs from the peripheral blood of WT recipient mice treated with CLs (CL ×2) or PBS liposomes (PBS ×2) as in panel B. Data for WT recipient mice injected with WT RBCs but not treated with liposomes (WT RBCs [no treatment]) are also revealed. The data in the lower panel are a more detailed representation of those in the upper panel. All data are means ± SEM for 3 recipient mice. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001 by Student t test (A-B) or by 2-way repeated-measures ANOVA with Tukey’s multiple-comparison test (C-D). NS, not significant.

Two injections of CLs abolish the rapid elimination of transfused Cd47−/− RBCs from WT recipient mice. (A) Representative flow cytometric plots of RPMs (upper left panel) and cDCs (lower left panel) and the percentage of RPMs among total splenic cells (upper right panel) and that of cDCs among total splenic cells (lower right panel) on day 7 after treatment of mice with 50 μL of CLs (CL ×1) or PBS liposomes (PBS ×1) on day 0. Data in the right panels are means ± SEM for 4 mice. (B) Representative flow cytometric plots of RPMs (upper left panel) and cDCs (lower left panel) and the percentage of RPMs among total splenic cells (upper right panel) and that of cDCs among total splenic cells (lower right panel) on day 2 after treatment of mice with 100 μL of CLs (CL ×2) or PBS liposomes (PBS ×2) on day 0 and day 1. Data in the right panels are means ± SEM for 3 mice. (C) Clearance of transfused Cd47−/− RBCs from peripheral blood of WT recipient mice treated with CLs (CL ×1) or PBS liposomes (PBS ×1) as in panel A. Data for WT recipient mice injected with WT RBCs but not treated with liposomes (WT RBCs [no treatment]) are also revealed. The data in the lower panel are a more detailed representation of those in the upper panel. All data are means ± SEM for 3 recipient mice. (D) Clearance of transfused Cd47−/− RBCs from the peripheral blood of WT recipient mice treated with CLs (CL ×2) or PBS liposomes (PBS ×2) as in panel B. Data for WT recipient mice injected with WT RBCs but not treated with liposomes (WT RBCs [no treatment]) are also revealed. The data in the lower panel are a more detailed representation of those in the upper panel. All data are means ± SEM for 3 recipient mice. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001 by Student t test (A-B) or by 2-way repeated-measures ANOVA with Tukey’s multiple-comparison test (C-D). NS, not significant.

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