Transfusion of Cd47^−/− RBCs into WT mice induces cDC activation in parallel with incorporation of the transferred cells into RPMs and their clearance from the bloodstream. (A) Clearance of WT or Cd47^−/− RBCs from peripheral blood of WT recipient mice. Data are means ± standard error of the mean (SEM) for 3 recipient mice. (B) Representative flow cytometric plots (left panel) and the percentage of CFSE-positive RPMs among total RPMs (right panel) for WT recipient mice at 2 hours after IV injection of CFSE-labeled WT or Cd47^−/− RBCs. Data for mice that were not injected with RBCs are also revealed (RBC iv[−]). Data in the right panel are means ± SEM for 3 recipient mice. (C-E) Representative flow cytometric analysis of CD86 (C), CCR7 (D), and MHCII (E) expression (left panels) and quantitative data for MFI (right panels) for cDC subsets of WT recipient mice at 2 hours after IV injection of CFSE-labeled WT or Cd47^−/− RBCs. Data for mice that were not injected with RBCs are also revealed (RBC iv[−]). Data in the right panels are means ± SEM for 3 recipient mice. ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001 by 2-way repeated-measures analysis of variance (ANOVA) with Tukey’s multiple-comparison test (A) or by 1-way ANOVA with Tukey’s multiple-comparison test (B-E). MFI, mean fluorescence intensity.