RNA-sequencing reveals features of decreased self-renewal and proteotoxic stress in CD99 Gt/Gt HSCs. (A) GSEA of key biological processes enriched or depleted between CD99 Gt/Gt and WT HSCs. (B) Venn diagram of DEGs (P < .01) between CD99 Gt/Gt and WT HSCs, or CD99 Gt/Gt and WT GMPs. (C) GSEA of biological processes identified to be enriched or depleted between CD99 Gt/Gt and WT GMPs. FDR, false discovery rate; NES, normalized enrichment score.