Murine Slc25a38 deficiency phenotype. (A) Slc25a38 gene targeting strategy. (B) Germ line Slc25a38-deficient E14.5 embryos show extreme pallor compared with the control. (C) Perls iron-stained peripheral blood smears (top; original magnification, 1000×) and transmission electron micrographs (TEMs) of peripheral blood (bottom; original magnification, 17 700×) from E14.5 embryos demonstrating iron-positive granules in mutant nucleated fetal erythrocytes confirmed to be electron-dense material within mitochondria by TEM. (D) RBC indices of control Slc25a38fl/− and Slc25a38fl/−; Vav1-cre+ (Slc25a38BMKO) animals. (E) Perls iron-stained peripheral blood smears (top; original magnification, 1000×) and TEMs of peripheral blood (bottom; original magnification, 22 000×) from 8-week-old animals demonstrating iron-positive granules in mutant erythrocytes confirmed to be electron-dense material within mitochondria on TEM. (F-I) Spleen-to-body mass ratio (F), EPO (G), liver iron (H), and splenic iron (I) in 8-week-old animals. For all panels, n = 5 or 6 mixed males and females in each group, aged 8 weeks, fed conventional mouse chow. The dotted line in each panel indicates the average value in control animals. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001, compared with control animals.
Figure 4.

Murine Slc25a38 deficiency phenotype. (A) Slc25a38 gene targeting strategy. (B) Germ line Slc25a38-deficient E14.5 embryos show extreme pallor compared with the control. (C) Perls iron-stained peripheral blood smears (top; original magnification, 1000×) and transmission electron micrographs (TEMs) of peripheral blood (bottom; original magnification, 17 700×) from E14.5 embryos demonstrating iron-positive granules in mutant nucleated fetal erythrocytes confirmed to be electron-dense material within mitochondria by TEM. (D) RBC indices of control Slc25a38fl/− and Slc25a38fl/−; Vav1-cre+ (Slc25a38BMKO) animals. (E) Perls iron-stained peripheral blood smears (top; original magnification, 1000×) and TEMs of peripheral blood (bottom; original magnification, 22 000×) from 8-week-old animals demonstrating iron-positive granules in mutant erythrocytes confirmed to be electron-dense material within mitochondria on TEM. (F-I) Spleen-to-body mass ratio (F), EPO (G), liver iron (H), and splenic iron (I) in 8-week-old animals. For all panels, n = 5 or 6 mixed males and females in each group, aged 8 weeks, fed conventional mouse chow. The dotted line in each panel indicates the average value in control animals. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001, compared with control animals.

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