Characterization of the female erythroid phenotypes of XLSA and XLPP mouse models. (A) Selected hematological indices for female control, XLSA, XLPP, and EPP mice maintained on conventional rodent chow at ages 5 and 8 weeks. (B) Representative photomicrographs of Perls iron-stained peripheral blood smears from heterozygous female carriers of XLSA or XLPP alleles at age 8 weeks. Arrows indicate siderocytes. The range of siderocyte frequency is indicated in the lower left of each image. Original magnification, 1000×. (C) RBC Zn-PPIX determined with by hematofluorometry. (D) RBC mf-PPIX MFI determined by flow cytometry. n = 5 to 9 mutant animals per group; n = 18 to 28 control animals. The dotted line in each panel indicates the average value in control animals. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001, compared with control animals at the same age. ^#P < .05; ^##P < .01; ^###P < .001; ^####P < .0001, compared with the same mutant animals at 8 weeks. CTL, control; MCV, mean corpuscular volume.