Platelet PS exposure is critical to fibrin accumulation during hemostatic plug formation. Mice expressing CypD in platelets and ECs (EC:WT; P:WT), mice lacking CypD in platelets only (EC:WT; P:CypD−/−), mice expressing CypD in platelets only (EC:CypD−/−; P:WT), or mice lacking CypD in both platelets and endothelium (EC:CypD−/−; P: E:CypD−/−) were generated by adoptive transfer technology (see “Methods”). (A) Surface view for accumulation of PS (aV, red) and platelets (gray) at the indicated time points during hemostatic plug formation in EC:WT; P:WT, EC:WT; P:CypD−/−, EC:CypD−/−; P:WT, or EC:CypD−/−; P: EC:CypD−/−(left to right) mice. (B) Fibrin (anti–fibrin-AF647, blue) and platelet (gray) accumulation at the indicated time points during hemostatic plug formation in the indicated mice. (C-E) SFI ± SEM over time for aV (C), fibrin (D), and platelets (E); n = 8 to 17 injuries in 3 to 4 mice. Statistical significance was determined for fluorescence signals of the last 3D stack acquired during the observation period (bar graphs on the right). ns, not significant; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. Scale bar indicates 50 μm.
Figure 6.

Platelet PS exposure is critical to fibrin accumulation during hemostatic plug formation. Mice expressing CypD in platelets and ECs (EC:WT; P:WT), mice lacking CypD in platelets only (EC:WT; P:CypD−/−), mice expressing CypD in platelets only (EC:CypD−/−; P:WT), or mice lacking CypD in both platelets and endothelium (EC:CypD−/−; P: E:CypD−/−) were generated by adoptive transfer technology (see “Methods”). (A) Surface view for accumulation of PS (aV, red) and platelets (gray) at the indicated time points during hemostatic plug formation in EC:WT; P:WT, EC:WT; P:CypD−/−, EC:CypD−/−; P:WT, or EC:CypD−/−; P: EC:CypD−/−(left to right) mice. (B) Fibrin (anti–fibrin-AF647, blue) and platelet (gray) accumulation at the indicated time points during hemostatic plug formation in the indicated mice. (C-E) SFI ± SEM over time for aV (C), fibrin (D), and platelets (E); n = 8 to 17 injuries in 3 to 4 mice. Statistical significance was determined for fluorescence signals of the last 3D stack acquired during the observation period (bar graphs on the right). ns, not significant; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. Scale bar indicates 50 μm.

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