TES evaluation of IS. (A) Location of unique IS relative to annotated canine genes (canFam3) detected using TES. The majority (93.8%) of unique IS were located in intergenic regions. For in-gene insertions, 206 of 355 (58%) fell within an intron and 149 of 355 (42%) in an exon. Upstream insertions are defined as intergenic regions 5′ of the closest gene, and downstream insertions are 3′ of the closest gene. In-gene insertions are defined as those located within the transcriptional unit. (B-D) Cumulative sequence counts of the top-10 IS were identified. (B) Most (10/16) samples exhibited integration profiles in which the top IS demonstrated frequencies <10% of the total sequences. Representative examples include VC-2 and FLO-1. (C) 4 of 16 samples had at least 1 IS with a frequency of >10% and <30% of the total IS sequence count. Representative examples include JU-1 and MZ-1. (D) 2 of 16 samples had at least 1 IS with a frequency of >30%. Both of these samples were obtained from nonresponding dogs (ANG-2 and MG-1). Refer to supplemental Figure 4 for top-10 IS data for all 16 samples analyzed. NR, nonresponder.