Neddylation inhibition increases PD-L1 expression in ATLL cells. (A) Cell surface expression of PD-L1 in KK1 and ST1 ATLL cells transduced with the indicated sgRNA by flow cytometry. (B) Immunoblot analysis of PD-L1, UBA3, NEDD8, NAE1, CUL3, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in sgAAVS1, sgUBA3, sgNEDD8, sgNAE1, and sgCUL3-transduced KK1 cells. Error bars represent the mean with SEM of replicates. ∗P < .05; ∗∗P < .01; Welch 2-sample t test. All experiments were repeated at least twice.
Figure 3.

Neddylation inhibition increases PD-L1 expression in ATLL cells. (A) Cell surface expression of PD-L1 in KK1 and ST1 ATLL cells transduced with the indicated sgRNA by flow cytometry. (B) Immunoblot analysis of PD-L1, UBA3, NEDD8, NAE1, CUL3, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in sgAAVS1, sgUBA3, sgNEDD8, sgNAE1, and sgCUL3-transduced KK1 cells. Error bars represent the mean with SEM of replicates. ∗P < .05; ∗∗P < .01; Welch 2-sample t test. All experiments were repeated at least twice.

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