The 13-gene p53 score is predictive of overall survival. (A) The score discriminates patient samples according to TP53 status. The score was calculated in 38 patient samples characterized by DGE-seq. Del17p and TP53 sequencing were determined by FISH and Sanger sequencing of complementary DNA products, respectively (supplemental Table 6).²⁵ Statistical analysis was performed using the Kruskal-Wallis test with multiple comparisons. (B) The p53 score is predictive of overall survival (OS) of 139 CASSIOPEA patients. Groups of patients were determined according to the score using the K-means method (supplemental Figure 3A). Left graph represents the OS of the 4 groups. Middle and right graphs represent the frequency of patients classified as high risk or with del17p in each K-means groups. Statistical significance was determined using the log-rank (Mantel-Cox) test or χ² test. (C) OS of 764 MMRF-coMMpass patients according to p53 score. Groups were determined using the K-means method (supplemental Figure 3B). Left graph represents the OS of the 4 groups. Middle graph represents the p53 score in groups separated according to the presence of TP53 deletion or/and mutation in 684 patients, and the right graph represents their OS. Variant allele frequency thresholds were set up at < −0.5 for deletion and >0.35 for mutation. Statistical significance was determined using the log-rank (Mantel-Cox) test. ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.