SAXS analysis of nanodisc-bound Xase complex. (A) Kratky plot analysis. (B) Scatter curves for experimental data (blue) and theoretical model (black, χ² = 0.25). (C) Pairwise distribution function. Inset depicts experimental data (blue) and an indirect Fourier transform of the P(r) distribution plot (black), showing strong agreement between both sets of data. (D) Ab initio bead models calculated using DAMAVER (faded) and DAMFILT (solid) and aligned by SUPCOMB. (E) Alignment of modeled tenase complex bound to a lipid nanodisc in sphere representation (fVIIIa heavy chain: dark blue; fVIIIa light chain: cyan; fIXa heavy chain: light green; fIXa light chain: dark green; scaffold protein: red; lipids: light orange) with the calculated ab initio envelope (mesh) by SUPCOMB. (F) Model of the nanodisc-bound Xase complex (fVIIIa heavy chain: dark blue; fVIIIa light chain: cyan; fIXa heavy chain: light green; fIXa light chain: dark green; scaffold protein: red; lipids: light orange).