Proteomic changes induced by loss of the miR-15a/16-1 cluster in GC B cells. (A) Schematic design of the proteomic analysis. GC B cells were flow sorted from WT (n = 5) and KO (n = 5) mice 10 days after immunized with SRBCs. Extracted proteins were trypsinized and subjected to tandem mass tag–based liquid chromatography coupled with tandem MS, followed by in silico analyses. (B) Differentially expressed proteins between WT and KO mice at FDR < 0.1. Proteins upregulated in KO compared with WT mice are indicated in red; downregulated in blue. (C) miR target enrichment in proteins upregulated in GC B cells from KO compared with WT mice determined using MIENTURNET. Color scale represents FDR value. For simplicity, miR family names were abbreviated. (D) Network of enriched pathways and processes in proteins differentially expressed between GC B cells from KO and WT mice using Metascape. Each node represents an enriched term and is colored by functional category. (E) GSEA of "Hallmark" gene sets in proteomes of GC B cells from KO compared with WT mice. Bubble size represents enrichment signal strength calculated as the GSEA leading edge signal. Gene sets enriched in KO and WT cells are shown in red and blue, respectively. EMT, epithelial-mesenchymal transition; IFN, interferon; logFC, log fold change; ncRNA, noncoding RNA; NES, normalized enrichment score; OxPhos, oxidative phosphorylation; tRNA, transfer RNA.