Comparison of Various Support Matrices and Transduction of Leukemia Cells
| Transduction Method . | % Cells Expressing EGFP . | |
|---|---|---|
| Nalm-6 . | Reh . | |
| PG13-LN3-150 | 0 | 0 |
| PG13-LEGFP3-150 + protamine sulfate (4 μg/mL) | 7.6 | 0.1 |
| PG13-LEGFP3-150 + protamine sulfate (4 μg/mL) on bone marrow stroma | 13.7 | 0.9 |
| PG13-LEGFP3-150 on fibronectin (50 μg/mL) | 42.1 | 1 |
| PG13-LEGFP3-150 on BSA (2%) | 5.1 | 0.1 |
| Transduction Method . | % Cells Expressing EGFP . | |
|---|---|---|
| Nalm-6 . | Reh . | |
| PG13-LN3-150 | 0 | 0 |
| PG13-LEGFP3-150 + protamine sulfate (4 μg/mL) | 7.6 | 0.1 |
| PG13-LEGFP3-150 + protamine sulfate (4 μg/mL) on bone marrow stroma | 13.7 | 0.9 |
| PG13-LEGFP3-150 on fibronectin (50 μg/mL) | 42.1 | 1 |
| PG13-LEGFP3-150 on BSA (2%) | 5.1 | 0.1 |
MOI of 20.