Table 1. PCR primer sequences used in... | American Society of Hematology

Table 1.

PCR primer sequences used in ChIP

	Forward primer	Reverse primer
hTERT Buffer G^*	GGTTACCCCACAGCCTAGGCCGATTC	CAGGGCTTCCCACGTGCGCAGCAGGACGCAGC
C17 Buffer L	CTGTGCAGAAAGAAATTTCTTG	CATGGTGCCAGGCGCTGGTG
c-Myc Buffer A	ACCCTCCCCACCCTCCCCATAAG	GCCTCTGAGAAGCCCTGCCCTTC
hTERT control Buffer A†	GGTAATGAAGTGGTGTGCAGGAAA	TGGCTGAAAGCAGCCTCATCTCTC
C17 control Buffer E	CTTTAACTTGGCTGCTTAGAC	CATAGTTTAGAGGGGACTCC
c-Myc control Buffer A	ACATCAACCCCATGAAGGAGATAC	GCCTGACCAGGCTTAGATGTTGAG

	Forward primer	Reverse primer
hTERT Buffer G^*	GGTTACCCCACAGCCTAGGCCGATTC	CAGGGCTTCCCACGTGCGCAGCAGGACGCAGC
C17 Buffer L	CTGTGCAGAAAGAAATTTCTTG	CATGGTGCCAGGCGCTGGTG
c-Myc Buffer A	ACCCTCCCCACCCTCCCCATAAG	GCCTCTGAGAAGCCCTGCCCTTC
hTERT control Buffer A†	GGTAATGAAGTGGTGTGCAGGAAA	TGGCTGAAAGCAGCCTCATCTCTC
C17 control Buffer E	CTTTAACTTGGCTGCTTAGAC	CATAGTTTAGAGGGGACTCC
c-Myc control Buffer A	ACATCAACCCCATGAAGGAGATAC	GCCTGACCAGGCTTAGATGTTGAG

Fail-safe buffers were used, as indicated in “Materials and methods”

Control indicates a DNA region 5 to 6 kb away from the promoter