Primers used for the analysis of the Fas gene
. | Primer sequences . | Size of PCR product, bp . | Annealing temperature, °C . |
|---|---|---|---|
| 5′UTR - Exon 1 | 322 | 64 | |
| Primer forward | 5′–AGCTCGTCTCTGATCTCGC–3′ | ||
| Primer reverse | 5′–TATCCCCGGGACTAAGACG–3′ | ||
| Exon 2 | 291 | 58 | |
| Primer forward | 5′–ACACTGGTTTACACGTTGCT–3′ | ||
| Primer reverse | 5′–CTATGTGCTACTCCTAACTG–3′ | ||
| Exon 3 | 304 | 58 | |
| Primer forward | 5′–GTCATCCCTCTATACTTCCC–3′ | ||
| Primer reverse | 5′–ACAGTAGGCCCCAATTTCAA–3′ | ||
| Exon 4 | 272 | 63 | |
| Primer forward | 5′–AATCCATGCAGCTCCTGCC–3′ | ||
| Primer reverse | 5′–AGTCAGTGTTACTTCCCTAGGA–3′ | ||
| Exon 5-exon 6 | 388 | 58 | |
| Primer forward | 5′–AAGGAATTATTCTGCCAGGC–3′ | ||
| Primer reverse | 5′–ATCTTTGTGAACTACTTCCCA–3′ | ||
| Exon 7 | 216 | 58 | |
| Primer forward | 5′–GAAAGTATGTTCTCACATGCA–3′ | ||
| Primer reverse | 5′–AGCCAAATCACTAATTTCTCTA–3′ | ||
| Exon 8 | 279 | 58 | |
| Primer forward | 5′–GGCCGGAACCTTTCAGAATA–3′ | ||
| Primer reverse | 5′–ATTGGCCTATTACTCTAAAGG–3′ | ||
| Exon 9 | 481 | 63 | |
| Primer forward | 5′–CATGGTTTTCACTAATGGG–3′ | ||
| Primer reverse | 5′–CAAGCAGTATTTACAGCCAGC–3′ |
. | Primer sequences . | Size of PCR product, bp . | Annealing temperature, °C . |
|---|---|---|---|
| 5′UTR - Exon 1 | 322 | 64 | |
| Primer forward | 5′–AGCTCGTCTCTGATCTCGC–3′ | ||
| Primer reverse | 5′–TATCCCCGGGACTAAGACG–3′ | ||
| Exon 2 | 291 | 58 | |
| Primer forward | 5′–ACACTGGTTTACACGTTGCT–3′ | ||
| Primer reverse | 5′–CTATGTGCTACTCCTAACTG–3′ | ||
| Exon 3 | 304 | 58 | |
| Primer forward | 5′–GTCATCCCTCTATACTTCCC–3′ | ||
| Primer reverse | 5′–ACAGTAGGCCCCAATTTCAA–3′ | ||
| Exon 4 | 272 | 63 | |
| Primer forward | 5′–AATCCATGCAGCTCCTGCC–3′ | ||
| Primer reverse | 5′–AGTCAGTGTTACTTCCCTAGGA–3′ | ||
| Exon 5-exon 6 | 388 | 58 | |
| Primer forward | 5′–AAGGAATTATTCTGCCAGGC–3′ | ||
| Primer reverse | 5′–ATCTTTGTGAACTACTTCCCA–3′ | ||
| Exon 7 | 216 | 58 | |
| Primer forward | 5′–GAAAGTATGTTCTCACATGCA–3′ | ||
| Primer reverse | 5′–AGCCAAATCACTAATTTCTCTA–3′ | ||
| Exon 8 | 279 | 58 | |
| Primer forward | 5′–GGCCGGAACCTTTCAGAATA–3′ | ||
| Primer reverse | 5′–ATTGGCCTATTACTCTAAAGG–3′ | ||
| Exon 9 | 481 | 63 | |
| Primer forward | 5′–CATGGTTTTCACTAATGGG–3′ | ||
| Primer reverse | 5′–CAAGCAGTATTTACAGCCAGC–3′ |