Table 1. Real-time PCR reaction... | American Society of Hematology

Table 1.

Real-time PCR reaction information

Gene name	Primer sequence	Amplicon, bp	MgCl₂ concentration, mM
GITR	Forward– ATTGTGTTTGGCTTCCG	238	4
	Reverse– GCTGGACTGTGGTTAGG
FoxP3	Forward– ATTTACTCAACCCAAACCCT	156	4
	Reverse– TGTGTGATAGTGCCCGT
HPRT	Forward– ACAGCCCCAAAATGGTTAAGG	324	3
	Reverse– TCTGGGGACGCAGCAACTGAC
Ribosomal protein S8	Forward– AAACGAAAACCCTACCAC	142	3
	Reverse– AAGTTCCCCACATCCAA

Gene name	Primer sequence	Amplicon, bp	MgCl₂ concentration, mM
GITR	Forward– ATTGTGTTTGGCTTCCG	238	4
	Reverse– GCTGGACTGTGGTTAGG
FoxP3	Forward– ATTTACTCAACCCAAACCCT	156	4
	Reverse– TGTGTGATAGTGCCCGT
HPRT	Forward– ACAGCCCCAAAATGGTTAAGG	324	3
	Reverse– TCTGGGGACGCAGCAACTGAC
Ribosomal protein S8	Forward– AAACGAAAACCCTACCAC	142	3
	Reverse– AAGTTCCCCACATCCAA