Table 2. Characterization of the... | American Society of Hematology

Table 2.

Characterization of the immortalized human PBMCs by immunocytochemistry

	Infecting mycoplasma	Cell surface marker					Immunoglobulin clonality	EBV LMP1
Sample ID	Infecting mycoplasma		LCA	CD20	CD3		Immunoglobulin clonality	EBV LMP1
K2267-Mi^*	Mi	+	+	-	Monoclonal (K)	+
K4117-Mi^*	Mi	+	+	-	Monoclonal (K)	+
K4119-Mi^*	Mi	+	+	-	Polyclonal	+
K4123-Mi^*	Mi	+	+	-	Monoclonal (L)	+
K4413-Mi^*	Mi	+	+	-	Monoclonal (K)	+
K4414-Mi†	Mi	+	+	-	Polyclonal	+
K4420-Mi†	Mi	+	+	-	Monoclonal (L)	+
K4423-Mi^*	Mi	+	+	-	Monoclonal (K)	+
K471-Mi^*	Mi	+	+	-	Polyclonal	+
K611-Mi^*	Mi	+	+	-	Polyclonal	+
K613-Mi^*	Mi	+	+	-	Polyclonal	+
K351-Mi†	Mi	+	+	-	Polyclonal	+
K352-Mi†	Mi	+	+	-	Polyclonal	+
K933-Mi†	Mi	+	+	-	Polyclonal	+
K1277-Mi†	Mi	+	+	-	Monoclonal (K)	+
K1025-Mi†	Mi	+	+	-	Monoclonal (L)	+
K1014-Mi†	Mi	+	+	-	Polyclonal	+
K1025-PG†	PG18	+	+	-	Polyclonal	+
K1023-PG†	PG18	+	+	-	Polyclonal	+
K1012-PG^*	PG18	+	+	-	Monoclonal (L)	+
K351-00	Spontaneous	+	+	-	Polyclonal	+
K352-00	Spontaneous	+	+	-	Polyclonal	+
K969-00	Spontaneous	+	+	-	Polyclonal	+
K1025-00	Spontaneous	+	+	-	Monoclonal (K)	+

	Infecting mycoplasma	Cell surface marker					Immunoglobulin clonality	EBV LMP1
Sample ID	Infecting mycoplasma		LCA	CD20	CD3		Immunoglobulin clonality	EBV LMP1
K2267-Mi^*	Mi	+	+	-	Monoclonal (K)	+
K4117-Mi^*	Mi	+	+	-	Monoclonal (K)	+
K4119-Mi^*	Mi	+	+	-	Polyclonal	+
K4123-Mi^*	Mi	+	+	-	Monoclonal (L)	+
K4413-Mi^*	Mi	+	+	-	Monoclonal (K)	+
K4414-Mi†	Mi	+	+	-	Polyclonal	+
K4420-Mi†	Mi	+	+	-	Monoclonal (L)	+
K4423-Mi^*	Mi	+	+	-	Monoclonal (K)	+
K471-Mi^*	Mi	+	+	-	Polyclonal	+
K611-Mi^*	Mi	+	+	-	Polyclonal	+
K613-Mi^*	Mi	+	+	-	Polyclonal	+
K351-Mi†	Mi	+	+	-	Polyclonal	+
K352-Mi†	Mi	+	+	-	Polyclonal	+
K933-Mi†	Mi	+	+	-	Polyclonal	+
K1277-Mi†	Mi	+	+	-	Monoclonal (K)	+
K1025-Mi†	Mi	+	+	-	Monoclonal (L)	+
K1014-Mi†	Mi	+	+	-	Polyclonal	+
K1025-PG†	PG18	+	+	-	Polyclonal	+
K1023-PG†	PG18	+	+	-	Polyclonal	+
K1012-PG^*	PG18	+	+	-	Monoclonal (L)	+
K351-00	Spontaneous	+	+	-	Polyclonal	+
K352-00	Spontaneous	+	+	-	Polyclonal	+
K969-00	Spontaneous	+	+	-	Polyclonal	+
K1025-00	Spontaneous	+	+	-	Monoclonal (K)	+

K indicates kappa light chain; and L, lambda light chain.

Cultures found to be free of mycoplasmas after immortalization following mycoplasmal infection

Immortalized cultures that still retained the infecting mycoplasmas