Detection of BCL-1 leukemic cells in spleen of BMT recipient mice by adoptive transfer to secondary BALB/c recipient mice
| Group . | Donors . | Recipients . | |||||
|---|---|---|---|---|---|---|---|
| Days after BCL-1/BMT . | N . | No. cells . | N . | No. of survivors/total mice . | MST (d) . | Spleen weight at time of death (mg) . | |
| A. Syngeneic BMT + BCL-1 | 11 | 8 | 1 × 106 | 9 | 0/9 | 15 | 1610 ± 70 |
| B. Allogeneic BMT + BCL-1 | 11 | 7 | 1 × 106 | 9 | 8/9 | > 100 | 125 ± 6 |
| 23 | 5 | 1 × 106 | 8 | 8/8 | > 100 | 123 ± 5 | |
| C. Allogeneic BMT + BCL-1 → WHI-P131 | 11 | 7 | 1 × 106 | 9 | 9/9 | > 100 | 122 ± 3 |
| 23 | 6 | 1 × 106 | 7 | 7/7 | > 100 | 137 ± 12 | |
| > 60 | 9 | 0.5-1 × 106 | 9 | 9/9 | > 100 | 117 ± 6 | |
| D. Syngeneic BMT + BCL-1 → WHI-P131 | 11 | 8 | 1 × 106 | 9 | 0/9 | 15 | 1500 ± 97 |
| Group . | Donors . | Recipients . | |||||
|---|---|---|---|---|---|---|---|
| Days after BCL-1/BMT . | N . | No. cells . | N . | No. of survivors/total mice . | MST (d) . | Spleen weight at time of death (mg) . | |
| A. Syngeneic BMT + BCL-1 | 11 | 8 | 1 × 106 | 9 | 0/9 | 15 | 1610 ± 70 |
| B. Allogeneic BMT + BCL-1 | 11 | 7 | 1 × 106 | 9 | 8/9 | > 100 | 125 ± 6 |
| 23 | 5 | 1 × 106 | 8 | 8/8 | > 100 | 123 ± 5 | |
| C. Allogeneic BMT + BCL-1 → WHI-P131 | 11 | 7 | 1 × 106 | 9 | 9/9 | > 100 | 122 ± 3 |
| 23 | 6 | 1 × 106 | 7 | 7/7 | > 100 | 137 ± 12 | |
| > 60 | 9 | 0.5-1 × 106 | 9 | 9/9 | > 100 | 117 ± 6 | |
| D. Syngeneic BMT + BCL-1 → WHI-P131 | 11 | 8 | 1 × 106 | 9 | 0/9 | 15 | 1500 ± 97 |
Splenocytes were obtained at the indicated time points from lethally irradiated F1 mice reconstituted with (A) syngeneic BM/S grafts and injected with BCL-1 cells, (B) allogeneic BM/S grafts and injected with BCL-1 cells, (C) same as group B, but treated with WHI-P131, (D) same as group A but treated with WHI-P131.