Binding kinetics of isolated scFvKd, kon, andkoff were determined by surface plasmon resonance as described in “Materials and methods”
| scFv . | Kon(M−1s−1) . | Koff(s−1) . | Kd (nM) . |
|---|---|---|---|
| EL5 | 1.6 × 104 | 3.6 × 10−3 | 226 |
| EL9 | 3.1 × 104 | 1.3 × 10−3 | 42 |
| EL14 | 3.7 × 104 | 4.2 × 10−5 | 1.1 |
| WR1 | 2.7 × 104 | 3.9 × 10−4 | 14 |
| WR8 | 1.2 × 104 | 5.3 × 10−5 | 4.2 |
| WR10 | 4.1 × 104 | 4.9 × 10−4 | 12 |
| WR11 | 8.8 × 103 | 4.2 × 10−5 | 4.8 |
| WR13 | 3.0 × 105 | 6.4 × 10−4 | 2.1 |
| WR16 | 1.6 × 104 | 1.9 × 10−3 | 115 |
| scFv . | Kon(M−1s−1) . | Koff(s−1) . | Kd (nM) . |
|---|---|---|---|
| EL5 | 1.6 × 104 | 3.6 × 10−3 | 226 |
| EL9 | 3.1 × 104 | 1.3 × 10−3 | 42 |
| EL14 | 3.7 × 104 | 4.2 × 10−5 | 1.1 |
| WR1 | 2.7 × 104 | 3.9 × 10−4 | 14 |
| WR8 | 1.2 × 104 | 5.3 × 10−5 | 4.2 |
| WR10 | 4.1 × 104 | 4.9 × 10−4 | 12 |
| WR11 | 8.8 × 103 | 4.2 × 10−5 | 4.8 |
| WR13 | 3.0 × 105 | 6.4 × 10−4 | 2.1 |
| WR16 | 1.6 × 104 | 1.9 × 10−3 | 115 |