Differential effect of several treatments on the agonist-induced aggregation, adhesion, and fibrinogen binding to CHO cells coexpressing the human PAF and fibrinogen receptors
| . | Aggregation . | Adhesion . | Fg binding (%) . |
|---|---|---|---|
| PAF ant (200 nM) | − | − | ND |
| PTX (200 ng/mL) | + | − | ND |
| RGDS (1 mM) | − | − | 0 |
| H7 (25 μM) | + | − | 75 |
| Staurosporine (0.5 μM) | + | − | 73 |
| BIM-I (1 μM) | + | − | 91 |
| EDTA (5 mM) | − | − | 0 |
| W7 (20 μM) | − | + | 22 |
| BAPTA-AM (100 μM) | − | + | 0 |
| Genistein (100 μM) | + | + | 100 |
| PD98059 (50 μM) | + | + | ND |
| Ly294002 (20 μM) | + | + | ND |
| db-cAMP (500 μM) | + | + | ND |
| . | Aggregation . | Adhesion . | Fg binding (%) . |
|---|---|---|---|
| PAF ant (200 nM) | − | − | ND |
| PTX (200 ng/mL) | + | − | ND |
| RGDS (1 mM) | − | − | 0 |
| H7 (25 μM) | + | − | 75 |
| Staurosporine (0.5 μM) | + | − | 73 |
| BIM-I (1 μM) | + | − | 91 |
| EDTA (5 mM) | − | − | 0 |
| W7 (20 μM) | − | + | 22 |
| BAPTA-AM (100 μM) | − | + | 0 |
| Genistein (100 μM) | + | + | 100 |
| PD98059 (50 μM) | + | + | ND |
| Ly294002 (20 μM) | + | + | ND |
| db-cAMP (500 μM) | + | + | ND |
The effect of the different agents on the PAF-induced aggregation and adhesion of CHO-αIIbβ3-PAFR cells was assessed as described in “Materials and methods.” Positive (+) or negative (−) signs indicate the presence or absence of adhesion or aggregation. Fibrinogen binding is expressed as percent of the Fg bound to PAF-stimulated cells in the absence of antagonists.
CHO indicates Chinese hamster ovary; PAF, platelet-activating factor; PAF ant, PAF antagonist; PTX, pertussis toxin; RGDS, Arg-Gly-Asp-Ser; EDTA, ethylenediaminetetraacetic acid; ND, not determined.