Cytotoxic vs apoptotic effect of Rituxan homodimers on different cell lines
| Cells . | IC50 (×10−7 M)3-150 . | % Apoptotic cells3-151 . |
|---|---|---|
| Daudi | 6.0 ± 2.7 | 32.2 |
| Raji | 3.4 ± 2.0 | 19.5 |
| Ramos | 0.23 ± 0.2 | 32.3 |
| DHL-4 | 4.8 ± 1.0 | 30.3 |
| Namalwa | 2.2 ± 1.0 | 23.9 |
| Namalwa/MDR-1 | 3.0 ± 1.0 | 25.7 |
| Jurkat | >10 | 0.6 |
| Cells . | IC50 (×10−7 M)3-150 . | % Apoptotic cells3-151 . |
|---|---|---|
| Daudi | 6.0 ± 2.7 | 32.2 |
| Raji | 3.4 ± 2.0 | 19.5 |
| Ramos | 0.23 ± 0.2 | 32.3 |
| DHL-4 | 4.8 ± 1.0 | 30.3 |
| Namalwa | 2.2 ± 1.0 | 23.9 |
| Namalwa/MDR-1 | 3.0 ± 1.0 | 25.7 |
| Jurkat | >10 | 0.6 |
Cells were incubated for 24 hours at 37° with 1 to 100 μg Rituxan and pulsed with 3H-thymidine for 4 hours. IC50 values represent the concentration of mAbs necessary to kill 50% of cells (3 to 4 experiments carried out).
Cells were incubated for 24 hours at 37° with 10 μg Rituxan and then stained with FITC–Annexin V and propridium iodide. Data represent 1 experiment of 2 carried out.