Distribution of CD164 epitope classes in adult human tissues
| Tissue . | Monoclonal antibody class . | ||
|---|---|---|---|
| Class I 105A5 . | Class II 103B2/9E10 . | Class III N6B6 and 67D2 . | |
| Lymphoid system | |||
| Spleen | Red and white pulp positively labeled | Red pulp positive (sheathed capillaries); weak positivity of central arteriole in white pulp | Pan-reactive |
| Tonsil | Lymphoid cells and macrophages positive | Endothelium and some MECA-79+ high endothelial venules positive; scattered cells and basal layer epithelium positive | Pan-reactive |
| Thymus | Lymphocytes in cortex positive, medulla very weakly positive, and macrophages positively labeled | Endothelium, subcapsular epithelium, and macrophages positively labeled; high endothelial venules positive in hyperplastic thymus | Pan-reactive |
| Central nervous system | |||
| Brain | Cerebral capillaries positive | Cerebral capillaries positive | Cerebral capillaries positive |
| Spinal cord | Capillaries positive | Capillaries positive | Capillaries positive |
| Gastrointestinal tract | |||
| Colon | Infiltrating lymphocytes and macrophages positive; goblet cells and associated mucin negative; epithelium shows very weak or negligible staining | Vacuolar membrane in goblet cells positive, apical epithelium also labeled weakly; endothelium positive | Staining more homogeneous, exhibiting a more pan-reactive appearance; infiltrating lymphocytes, macrophages, and endothelia positive; vacuolar membranes of goblet cells positive; apical epithelium also labeled |
| Liver | Kupffer cells positively labeled | Lumenal staining of bile ductules but not bile ducts; hepatocytes negative | Hepatocytes and Kupffer cells positively labeled |
| Urinary system | |||
| Kidney | Weak labeling of tubules, glomeruli positive; mesengeal cells positive | Weak labeling of tubules, glomeruli negative; mesengeal cells positive | Very strong labeling of tubules and glomeruli; mesengeal, macrophage, and plasma cells positive |
| Circulatory system | |||
| Heart | Endothelium positive | Weak labeling of endothelium | Endothelium positive |
| Endocrine system | |||
| Adrenal cortex | Adrenal cortical cells positive | Adrenal cortical cells positive | Adrenal cortical cells positive |
| Pancreas | Islets of Langerhans labeled together with occasional cells strongly labeled | Exocrine acini positively labeled; islets of Langerhans negative | Pan-reactive |
| Salivary gland | Secretory lobules positive, excretory ducts negative | Secretory lobules positive; apical membrane of excretory ducts positive | Secretory lobules positive, excretory ducts negative and also clear labeling of apical membrane of secretory ducts |
| Thyroid gland | Cuboidal epithelium labeled; parathyroid weakly labeled | Cuboidal epithelium and endothelium positive; parafollicular cells and parathyroid negative | Thyroid cells labeled; parathyroid labeled strongly for N6B6 and weakly for 67D2 |
| Respiratory system | |||
| Lung | Macrophages weakly positive | Macrophages weakly positive | Macrophages strongly positive |
| Reproductive system | |||
| Placenta | Syncytiotrophoblasts positive | Occasional syncytiotrophoblasts positive | Syncytiotrophoblasts positive and mesenchymal cells positive |
| Testis | Leydig cells and seminiferous tubules positive | Weak labeling of cells in seminiferous tubules; Leydig cells negative | Pan-reactive |
| Skin | Basal layer and endothelium weakly positive | Basal layer and endothelium positive | Basal layer and endothelium positive |
| Tissue . | Monoclonal antibody class . | ||
|---|---|---|---|
| Class I 105A5 . | Class II 103B2/9E10 . | Class III N6B6 and 67D2 . | |
| Lymphoid system | |||
| Spleen | Red and white pulp positively labeled | Red pulp positive (sheathed capillaries); weak positivity of central arteriole in white pulp | Pan-reactive |
| Tonsil | Lymphoid cells and macrophages positive | Endothelium and some MECA-79+ high endothelial venules positive; scattered cells and basal layer epithelium positive | Pan-reactive |
| Thymus | Lymphocytes in cortex positive, medulla very weakly positive, and macrophages positively labeled | Endothelium, subcapsular epithelium, and macrophages positively labeled; high endothelial venules positive in hyperplastic thymus | Pan-reactive |
| Central nervous system | |||
| Brain | Cerebral capillaries positive | Cerebral capillaries positive | Cerebral capillaries positive |
| Spinal cord | Capillaries positive | Capillaries positive | Capillaries positive |
| Gastrointestinal tract | |||
| Colon | Infiltrating lymphocytes and macrophages positive; goblet cells and associated mucin negative; epithelium shows very weak or negligible staining | Vacuolar membrane in goblet cells positive, apical epithelium also labeled weakly; endothelium positive | Staining more homogeneous, exhibiting a more pan-reactive appearance; infiltrating lymphocytes, macrophages, and endothelia positive; vacuolar membranes of goblet cells positive; apical epithelium also labeled |
| Liver | Kupffer cells positively labeled | Lumenal staining of bile ductules but not bile ducts; hepatocytes negative | Hepatocytes and Kupffer cells positively labeled |
| Urinary system | |||
| Kidney | Weak labeling of tubules, glomeruli positive; mesengeal cells positive | Weak labeling of tubules, glomeruli negative; mesengeal cells positive | Very strong labeling of tubules and glomeruli; mesengeal, macrophage, and plasma cells positive |
| Circulatory system | |||
| Heart | Endothelium positive | Weak labeling of endothelium | Endothelium positive |
| Endocrine system | |||
| Adrenal cortex | Adrenal cortical cells positive | Adrenal cortical cells positive | Adrenal cortical cells positive |
| Pancreas | Islets of Langerhans labeled together with occasional cells strongly labeled | Exocrine acini positively labeled; islets of Langerhans negative | Pan-reactive |
| Salivary gland | Secretory lobules positive, excretory ducts negative | Secretory lobules positive; apical membrane of excretory ducts positive | Secretory lobules positive, excretory ducts negative and also clear labeling of apical membrane of secretory ducts |
| Thyroid gland | Cuboidal epithelium labeled; parathyroid weakly labeled | Cuboidal epithelium and endothelium positive; parafollicular cells and parathyroid negative | Thyroid cells labeled; parathyroid labeled strongly for N6B6 and weakly for 67D2 |
| Respiratory system | |||
| Lung | Macrophages weakly positive | Macrophages weakly positive | Macrophages strongly positive |
| Reproductive system | |||
| Placenta | Syncytiotrophoblasts positive | Occasional syncytiotrophoblasts positive | Syncytiotrophoblasts positive and mesenchymal cells positive |
| Testis | Leydig cells and seminiferous tubules positive | Weak labeling of cells in seminiferous tubules; Leydig cells negative | Pan-reactive |
| Skin | Basal layer and endothelium weakly positive | Basal layer and endothelium positive | Basal layer and endothelium positive |