Table 1. Oligonucleotide primers used... | American Society of Hematology

Table 1.

Oligonucleotide primers used for the construction of the cysteine mutants of SakSTAR

Variant	Primer	Code	Sequence
External	Sense	818A	5′-CAG GAA ACA GAA TTC AGG AG-3′
	Antisense	818D	5′-CAA AAC AGC CAA GCT TCA TTC ATT CAG C-3′
	Antisense	819D	5′-CAG GCT GAA AAT CTT CTC TCA TCC GCC
SakSTAR(K96C)	Sense	LJ452	5′-TAT GAT AAG AAT $\underset{̲}{TGC}$ AAA AAA GAA GAA-3′
	Antisense	LJ453	5′-TTC TTC TTT TTT $\underset{̲}{GCA}$ ATT CTT ATC ATA-3′
SakSTAR(K102C)	Sense	LJ454	5′-AAA AAG AAG AAA $CG \underset{̲}{T GC} T$ CTT TCC CTA-3′
	Antisense	LJ455	5′-TAG GGA AAG $A \underset{̲}{GC A} CG$ TTT CTT CTT TTT-3′
SakSTAR(K109C)	Sense	LJ456	5′-CCC TAT AAC AGA $A \underset{̲}{TG C} GG$ TTT TGT TGT CCC-3′
	Antisense	LJ457	5′-GGG ACA ACA AAA $CC \underset{̲}{G CA} T$ TCT GTT ATA GGG-3′
SakSTAR(K135C)	Sense	LJ337	5′-GAA ACA GAA TTC AGG AGG CCT CAT ATG TCG AGC TCA TTC GAC AAA GG-3′
SakSTAR(K135C), SakSTAR(K96C, K135C)	Antisense	LJ522	5′-CAG CCA AGC TTC ATT CAT TCA GCA TGC TGC AGT TAT $TT \underset{̲}{G CA} T$ TCT ATA ACA ACC TTG G-3′
SakSTAR(S3C)	Sense	LY459	5′-CCT CAT ATG TCA AGT $\underset{̲}{TGT}$ TTC GAC AAA GGA-3′
SakSTAR(S2C, S3C)	Sense	LY461	5′-CCT CAT ATG TCA $\underset{̲}{TGT} \underset{̲}{TGT}$ TTC GAC AAA GGA-3′

Variant	Primer	Code	Sequence
External	Sense	818A	5′-CAG GAA ACA GAA TTC AGG AG-3′
	Antisense	818D	5′-CAA AAC AGC CAA GCT TCA TTC ATT CAG C-3′
	Antisense	819D	5′-CAG GCT GAA AAT CTT CTC TCA TCC GCC
SakSTAR(K96C)	Sense	LJ452	5′-TAT GAT AAG AAT $\underset{̲}{TGC}$ AAA AAA GAA GAA-3′
	Antisense	LJ453	5′-TTC TTC TTT TTT $\underset{̲}{GCA}$ ATT CTT ATC ATA-3′
SakSTAR(K102C)	Sense	LJ454	5′-AAA AAG AAG AAA $CG \underset{̲}{T GC} T$ CTT TCC CTA-3′
	Antisense	LJ455	5′-TAG GGA AAG $A \underset{̲}{GC A} CG$ TTT CTT CTT TTT-3′
SakSTAR(K109C)	Sense	LJ456	5′-CCC TAT AAC AGA $A \underset{̲}{TG C} GG$ TTT TGT TGT CCC-3′
	Antisense	LJ457	5′-GGG ACA ACA AAA $CC \underset{̲}{G CA} T$ TCT GTT ATA GGG-3′
SakSTAR(K135C)	Sense	LJ337	5′-GAA ACA GAA TTC AGG AGG CCT CAT ATG TCG AGC TCA TTC GAC AAA GG-3′
SakSTAR(K135C), SakSTAR(K96C, K135C)	Antisense	LJ522	5′-CAG CCA AGC TTC ATT CAT TCA GCA TGC TGC AGT TAT $TT \underset{̲}{G CA} T$ TCT ATA ACA ACC TTG G-3′
SakSTAR(S3C)	Sense	LY459	5′-CCT CAT ATG TCA AGT $\underset{̲}{TGT}$ TTC GAC AAA GGA-3′
SakSTAR(S2C, S3C)	Sense	LY461	5′-CCT CAT ATG TCA $\underset{̲}{TGT} \underset{̲}{TGT}$ TTC GAC AAA GGA-3′

Numbering and orientation are relative to the mature staphylokinase polypeptide.