Primers, Annealing Temperature, Sizes, and Amplification Cycles for PCR Analysis
| Name . | Primer Pair . | Temperature (°C) . | Size (bp) . | Cycles . |
|---|---|---|---|---|
| m-Crg-2/IP-10 | ACCATGAACCCAAGTGCTGCCGTC | 64 | 311 | 32 |
| GCTTCACTCCAGTTAAGGAGCCCT | ||||
| m-IFN-γ | TGCGGCCTAGCTCTGAGACAATGA | 62 | 413 | 40 |
| TGAATGCTTGGCGCTGGACCTGTG | ||||
| m-Perforin | CTGAGAAGACCTATCAGGACC | 58 | 429 | 35 |
| ACCAAGTACTTCGACGTGACG | ||||
| m-Granzyme B | ACACGCTACAAGAGGTTGAGC | 55 | 295 | 35 |
| GCAGGATCCATGTTGCTTCTG | ||||
| m-GAPDH | GCCACCCAGAAGACTGTGGATGGC | 62 | 446 | 28 |
| CATGTAGGCCATGAGGTCCACCAC |
| Name . | Primer Pair . | Temperature (°C) . | Size (bp) . | Cycles . |
|---|---|---|---|---|
| m-Crg-2/IP-10 | ACCATGAACCCAAGTGCTGCCGTC | 64 | 311 | 32 |
| GCTTCACTCCAGTTAAGGAGCCCT | ||||
| m-IFN-γ | TGCGGCCTAGCTCTGAGACAATGA | 62 | 413 | 40 |
| TGAATGCTTGGCGCTGGACCTGTG | ||||
| m-Perforin | CTGAGAAGACCTATCAGGACC | 58 | 429 | 35 |
| ACCAAGTACTTCGACGTGACG | ||||
| m-Granzyme B | ACACGCTACAAGAGGTTGAGC | 55 | 295 | 35 |
| GCAGGATCCATGTTGCTTCTG | ||||
| m-GAPDH | GCCACCCAGAAGACTGTGGATGGC | 62 | 446 | 28 |
| CATGTAGGCCATGAGGTCCACCAC |