Table 1. Efficiency of Gene Transfer... | American Society of Hematology

Table 1.

Efficiency of Gene Transfer Into NOD/SCID Repopulating Human Hematopoietic Cells

Experiment	Efficiency of Gene Transfer		No. of Transplanted Cells	Human Cell Engraftment (% CD45⁺ cells)	Gene-Marked Cells Derived From the BM of NOD/SCID Mice (% of all CD45⁺ cells)	LNGFR PCR Second Colonies (LNGFR⁺/total colonies)
Experiment	(% LNGFR⁺ cells)	(% CD34⁺LNGFR⁺/CD34⁺ cells)	No. of Transplanted Cells	Human Cell Engraftment (% CD45⁺ cells)		LNGFR PCR Second Colonies (LNGFR⁺/total colonies)
BM 3	80.8	79.6	1.25 × 10⁶	1.2	6.0
			2.5 × 10⁶	0.3	0
			2.5 × 10⁶	0	0
BM 5	83.1	78.6	3.0 × 10⁶	5.1	5.6
			4.2 × 10⁶	0	0
CB 1	70.8	74.2	1.25 × 10⁶	9.5	16.9	2/10
			1.25 × 10⁶	12.8	16.7	1/13
			1.25 × 10⁶	9.0	25.3	1/13
CB 3	83.3	81.3	1.3 × 10⁶	0	0
			1.3 × 10⁶	3.6	13.7	1/20
CB 4	84.2	80.8	0.5 × 10⁶	0	0
			0.5 × 10⁶	0	0
CB 5^-150	97.4	98.0	3.0 × 10⁶	33.3	56.6	9/20
			3.0 × 10⁶	0.9	15.1

Experiment	Efficiency of Gene Transfer		No. of Transplanted Cells	Human Cell Engraftment (% CD45⁺ cells)	Gene-Marked Cells Derived From the BM of NOD/SCID Mice (% of all CD45⁺ cells)	LNGFR PCR Second Colonies (LNGFR⁺/total colonies)
Experiment	(% LNGFR⁺ cells)	(% CD34⁺LNGFR⁺/CD34⁺ cells)	No. of Transplanted Cells	Human Cell Engraftment (% CD45⁺ cells)		LNGFR PCR Second Colonies (LNGFR⁺/total colonies)
BM 3	80.8	79.6	1.25 × 10⁶	1.2	6.0
			2.5 × 10⁶	0.3	0
			2.5 × 10⁶	0	0
BM 5	83.1	78.6	3.0 × 10⁶	5.1	5.6
			4.2 × 10⁶	0	0
CB 1	70.8	74.2	1.25 × 10⁶	9.5	16.9	2/10
			1.25 × 10⁶	12.8	16.7	1/13
			1.25 × 10⁶	9.0	25.3	1/13
CB 3	83.3	81.3	1.3 × 10⁶	0	0
			1.3 × 10⁶	3.6	13.7	1/20
CB 4	84.2	80.8	0.5 × 10⁶	0	0
			0.5 × 10⁶	0	0
CB 5^-150	97.4	98.0	3.0 × 10⁶	33.3	56.6	9/20
			3.0 × 10⁶	0.9	15.1

Abbreviations: BM, bone marrow; CB, umbilical cord blood.

Transduction was performed on CH-296–coated plate.