Recovery of Steady-State BM and Mobilized PB CD34+ Cells After Coculture on MS-5 Monolayer
| . | Recovery of CD34+Cells (%) . | |
|---|---|---|
| Nonadherent Fraction . | Adherent Fraction . | |
| Steady-state BM (N = 6) | 65 ± 1.4 | 32 ± 2.2 |
| Mobilized PB (N = 4) | 83 ± 2.1 | 17 ± 3.4 |
| . | Recovery of CD34+Cells (%) . | |
|---|---|---|
| Nonadherent Fraction . | Adherent Fraction . | |
| Steady-state BM (N = 6) | 65 ± 1.4 | 32 ± 2.2 |
| Mobilized PB (N = 4) | 83 ± 2.1 | 17 ± 3.4 |
1.0 × 105 steady-state BM and mobilized PB CD34+ cells were cultured on a monolayer of MS-5 cells. After a 1-hour incubation nonadherent cells were recovered by gentle agitation and then adherent cells by vigorous pipetting.
P < .001 by multiple t-test.