Effect of Cytochalasin B on Conjugate Formation
| . | bsAb (μg/mL) . | % of CD56+ Cells in Conjugates . | |
|---|---|---|---|
| . | . | Medium . | Cytochalasin B . |
| Experiment no. 1 | 0 | 18 | 18 |
| 0.6 | 28 | 28 | |
| 1.6 | 39 | 31 | |
| 3.2 | 52 | 39 | |
| Experiment no. 2 | 0 | 6 | 5 |
| 0.6 | 21 | 20 | |
| 1.6 | 32 | 34 | |
| 3.2 | 45 | 43 | |
| . | bsAb (μg/mL) . | % of CD56+ Cells in Conjugates . | |
|---|---|---|---|
| . | . | Medium . | Cytochalasin B . |
| Experiment no. 1 | 0 | 18 | 18 |
| 0.6 | 28 | 28 | |
| 1.6 | 39 | 31 | |
| 3.2 | 52 | 39 | |
| Experiment no. 2 | 0 | 6 | 5 |
| 0.6 | 21 | 20 | |
| 1.6 | 32 | 34 | |
| 3.2 | 45 | 43 | |
Activated LD-PBL were surface labeled with PE–anti-CD56 and mixed with TNP-MC1 cells in the presence of the indicated concentrations of anti-CD44(Fab) × anti-DNP(Fab) bsAb for 30 minutes at 37°C with either 0.5 μg/mL (experiment no. 1) or 0.125 μg/mL (experiment no. 2) cytochalasin B. Conjugates were detected as CD56+ cells having forward light scatter greater or equal to that of the TNP-MC1 cells.