ODNs Used in This Study
| ODN . | Sequence (5′-3′) . | Half-Life (Hour) . | |
|---|---|---|---|
| . | . | 2.5% NHS . | 10% FCS . |
| PO1 | GAAGGGCTTCTTCCTTAT | 22 ± 3 | <1 |
| PO2 | GAAGGGCTTCTTCCTTCT | 38 ± 4 | <1 |
| PO3 | GAAGGGCTTCTTCCTTGT | 19 ± 1 | |
| PO4 | GAAGGGCTTCTTCCTTTT | 17 ± 1 | |
| PO5 | GAAGGGCTTCTTCCTTAA | ||
| PO6 | GAAGGGCTTCTTCCTCAT | 24 ± 1 | |
| PO7 | GAAGGGCTTCTTCCTGAT | 29 ± 2 | |
| PO8 | GAAGGGCTTCTTCCTTAC | 32 ± 1 | |
| PO9 | GAAGGGCTTCTTCCTTAG | 47 ± 2 | |
| PO10 | AGTCTTGCATCGTCTTGA | ||
| PO11 | ATTCTGTGAAGTCCTTGC | ||
| PO12 | ATAAGGAAGAAGCCCTTC | 37 ± 4 | |
| PO13 | TTTTTTTTTTTTTTTTTT | 12 ± 1 | |
| PO14 | CCCCCCCCCCCCCCCCCC | >72 | |
| PO/PS1 | GAAGGGCTTCTTCCTTA(Ps)T | >100 | 15 ± 1 |
| PO/PS2 | GAAGGGCTTCTTCCTT(pS)AT | 23 ± 4 | <1 |
| PO/PS3 | GAAGGGCTTCTTCCT(pS)TAT | 25 ± 2 | <1 |
| PS1 | CGCTGAAGGGCTTCTTCCTTATTGAT | ||
| PS2 | ATCAATAAGGAAGAAGCCCTTCAGCG | ||
| PS3 | CGCTGAAGGGCTTTTGAACTCTGCTT | ||
| PS4 | AAGCAGAGTTCAAAAGCCCTTCAGCG | ||
| PS5 | CGCTGAAGGGCTTCTTCCTTATTGCG | ||
| PS6 | ATCAATAAGGAAGAAGCCCTTCAGAT | ||
| ODN . | Sequence (5′-3′) . | Half-Life (Hour) . | |
|---|---|---|---|
| . | . | 2.5% NHS . | 10% FCS . |
| PO1 | GAAGGGCTTCTTCCTTAT | 22 ± 3 | <1 |
| PO2 | GAAGGGCTTCTTCCTTCT | 38 ± 4 | <1 |
| PO3 | GAAGGGCTTCTTCCTTGT | 19 ± 1 | |
| PO4 | GAAGGGCTTCTTCCTTTT | 17 ± 1 | |
| PO5 | GAAGGGCTTCTTCCTTAA | ||
| PO6 | GAAGGGCTTCTTCCTCAT | 24 ± 1 | |
| PO7 | GAAGGGCTTCTTCCTGAT | 29 ± 2 | |
| PO8 | GAAGGGCTTCTTCCTTAC | 32 ± 1 | |
| PO9 | GAAGGGCTTCTTCCTTAG | 47 ± 2 | |
| PO10 | AGTCTTGCATCGTCTTGA | ||
| PO11 | ATTCTGTGAAGTCCTTGC | ||
| PO12 | ATAAGGAAGAAGCCCTTC | 37 ± 4 | |
| PO13 | TTTTTTTTTTTTTTTTTT | 12 ± 1 | |
| PO14 | CCCCCCCCCCCCCCCCCC | >72 | |
| PO/PS1 | GAAGGGCTTCTTCCTTA(Ps)T | >100 | 15 ± 1 |
| PO/PS2 | GAAGGGCTTCTTCCTT(pS)AT | 23 ± 4 | <1 |
| PO/PS3 | GAAGGGCTTCTTCCT(pS)TAT | 25 ± 2 | <1 |
| PS1 | CGCTGAAGGGCTTCTTCCTTATTGAT | ||
| PS2 | ATCAATAAGGAAGAAGCCCTTCAGCG | ||
| PS3 | CGCTGAAGGGCTTTTGAACTCTGCTT | ||
| PS4 | AAGCAGAGTTCAAAAGCCCTTCAGCG | ||
| PS5 | CGCTGAAGGGCTTCTTCCTTATTGCG | ||
| PS6 | ATCAATAAGGAAGAAGCCCTTCAGAT | ||
Phosphodiester (PO1 TO PO14), chimeric phosphodiester/phosphorothioate (PO/PS1 to PO/PS3), and all-phosphorothioate (PS1 to PS6) ODNs used in this study. The position of the phosphorothioate linkage is indicated by “pS” in the chimeric ODN sequences. Half-lives of the full-length ODNs are given in hours and correspond to an incubation of the ODNs at a 25 μmol/L concentration and at 37°C in RPMI 1640 culture media containing 2.5% NHS or 10% FCS. Data are the mean of three experiments ± SD.