The protocol for immunization and treatment of C57BI/6 mice with F/AIPO and CD22/cal
. | Week . | . | . | . | . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Group . | 0 . | 2 . | 4 . | 4 + 5 d . | 6 . | 8 . | 12 . | 12 + 4 d . | 14 + 4 d . | 25 . | |||||||||
| 1. F/AIPO | B/V | B/V | B/CD22 | CD22 | B | B | B | RSV | B | B/RSV | |||||||||
| 2. F/AIPO | B/V | B/V | B/PBS | PBS | B | B | B | RSV | B | B/RSV | |||||||||
| 3. PBS | B | – | B/CD22 | CD22 | B | B | B | RSV | B | B/RSV | |||||||||
| 4. PBS | B | – | B/PBS | PBS | B | B | B | RSV | B | B/RSV | |||||||||
. | Week . | . | . | . | . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Group . | 0 . | 2 . | 4 . | 4 + 5 d . | 6 . | 8 . | 12 . | 12 + 4 d . | 14 + 4 d . | 25 . | |||||||||
| 1. F/AIPO | B/V | B/V | B/CD22 | CD22 | B | B | B | RSV | B | B/RSV | |||||||||
| 2. F/AIPO | B/V | B/V | B/PBS | PBS | B | B | B | RSV | B | B/RSV | |||||||||
| 3. PBS | B | – | B/CD22 | CD22 | B | B | B | RSV | B | B/RSV | |||||||||
| 4. PBS | B | – | B/PBS | PBS | B | B | B | RSV | B | B/RSV | |||||||||
B indicates bleed (for serum or flow cytometry); V vaccination (F/AIPO); CD22, CD22/cal; PBS, hosphate-buffered saline; RSV, challenge with RSF; and –, not applicable.