Table 1 Location of DNA regions and... | American Society of Hematology

Table 1

Location of DNA regions and oligonucleotide primer pairs for PCR amplification of genomic DNA for preparation of reporter plasmid constructs

DNA regions	Position*	5′-primer	3′-primer
−8.8	−10536: −6495	ATCTGGGAGAAGGAATGGCCAGCATT	TGGGCTCTCGTTAGCTCCTATGTTCTTTAGT
−5.5†	−6209: −4687	(none)
−3.8†	−3982: −3281	(none)
+2.0†	+1823: +2597	(none)
+5.8	+4602: +7196	ATTTCCCTTCTTCTCACTTTGGGTTTAATTTG	ATACTGGAGCAAGGAAAGGTCACCAGACAAT
+11.5	+10803: +12797	TGTTTACAGAAGCTTTGTTGCTAGTGGCCC	ATCAGCCCGACTGCTCCTGCAGACTCT

DNA regions	Position*	5′-primer	3′-primer
−8.8	−10536: −6495	ATCTGGGAGAAGGAATGGCCAGCATT	TGGGCTCTCGTTAGCTCCTATGTTCTTTAGT
−5.5†	−6209: −4687	(none)
−3.8†	−3982: −3281	(none)
+2.0†	+1823: +2597	(none)
+5.8	+4602: +7196	ATTTCCCTTCTTCTCACTTTGGGTTTAATTTG	ATACTGGAGCAAGGAAAGGTCACCAGACAAT
+11.5	+10803: +12797	TGTTTACAGAAGCTTTGTTGCTAGTGGCCC	ATCAGCCCGACTGCTCCTGCAGACTCT

Position is presented by location relative to the ATG translation start site of exon 1 in ABO on genomic DNA of NCBI reference sequence NT_035014.4.

†

DNA regions −5.5, −3.8, and +2.0 were prepared from human genomic clone HG-1.

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