Table 1 Comparison of the VEGF-mediated... | American Society of Hematology

Table 1

Comparison of the VEGF-mediated Egr-1 and Egr-3 expression signals

Name	Target	egr-1 mRNA reduction rate ± SD (%)	egr-3 mRNA reduction rate ± SD (%)
DMSO		0	0
KDR nAb.	KDR	−81 ± 6.4*	−87 ± 5.7*
LY294002	PI3K	(superinduced by 60-fold)	−73 ± 2.4*
PD98059	MEK1/2	−68 ± 2.3*	−78 ± 4.4*
SB203580	p38	(superinduced by 10-fold)	N.E.
Cyclosporine A	Calcineurin	N.E.	−75 ± 3.9*
BAPTA-AM	Ca²⁺	−87 ± 3.9	−99 ± 0.1*
GF109203X	PKCα, βI, βI, σ, γ, ϵ	−44 ± 1.9†	−99 ± 0.1*
Gö6976	PKCα, βI	−40 ± 5.4†	−99 ± 0.1*
Rottlerin	PKCσ, θ	(superinduced by 10-fold)	−59 ± 3.2*
SP600125	JNK	−95 ± 2.1*	−99 ± 0.1*
H-85	PKA	(superinduced by 6-fold)	−73 ± 9.7*
Cycloheximide	Protein biosynthesis	(superinduced by 120-fold)	(superinduced by 10-fold)

Name	Target	egr-1 mRNA reduction rate ± SD (%)	egr-3 mRNA reduction rate ± SD (%)
DMSO		0	0
KDR nAb.	KDR	−81 ± 6.4*	−87 ± 5.7*
LY294002	PI3K	(superinduced by 60-fold)	−73 ± 2.4*
PD98059	MEK1/2	−68 ± 2.3*	−78 ± 4.4*
SB203580	p38	(superinduced by 10-fold)	N.E.
Cyclosporine A	Calcineurin	N.E.	−75 ± 3.9*
BAPTA-AM	Ca²⁺	−87 ± 3.9	−99 ± 0.1*
GF109203X	PKCα, βI, βI, σ, γ, ϵ	−44 ± 1.9†	−99 ± 0.1*
Gö6976	PKCα, βI	−40 ± 5.4†	−99 ± 0.1*
Rottlerin	PKCσ, θ	(superinduced by 10-fold)	−59 ± 3.2*
SP600125	JNK	−95 ± 2.1*	−99 ± 0.1*
H-85	PKA	(superinduced by 6-fold)	−73 ± 9.7*
Cycloheximide	Protein biosynthesis	(superinduced by 120-fold)	(superinduced by 10-fold)

DMCO indicates dimethyl sulfoxide; JNK, c-jun N-terminal kinase; KDR, kinase domain receptor; MEK1/2, MAP kinase/ERK kinase; N.E., no effect; PI3K, phosphoinositide-3 kinase; PKA, protein kinase A; and PKC, protein kinase C.

Indicates significant reduction.

†

indicates mild reduction.

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