Table 3 Vascular phenotypes resulting... | American Society of Hematology

Table 3

Vascular phenotypes resulting from small-molecule treatment of Xenopus embryos (compounds affecting blood vessel development only)

Phenotype class/compound name	Pharmacologic class	Selectivity	ISV phenotype	VVN phenotype	PCV phenotype	Lymphatic phenotype	Embryonic phenotype
Defective vasculogenesis
Nocodazole	Cytoskeleton and ECM	Beta-tubulin	Impaired	Hypoplastic	Hypoplastic	NA	EDE
Podophyllotoxin	Cytoskeleton and ECM		Impaired	Hypoplastic	Hypoplastic	NA	EDE
2-Methoxyestradiol	Hormone	Estrogen	Impaired	Hypoplastic	Hypoplastic	NA	EDE
IC 261	Phosphorylation	CK-1delta/epsilon	Impaired	Hypoplastic	Hypoplastic	NA	EDE
NSC 95397	Phosphorylation	Cdc25	Impaired	Hypoplastic	Hypoplastic	NA	LET
Tyrphostin AG 494	Phosphorylation	EGFR	Impaired	Hypoplastic	Hypoplastic	NA	EDE
Meclofenamic acid sodium	Prostaglandin	COX/5-lipoxygenase	Impaired	Hypoplastic	Hypoplastic	NA	EDE
Defective angiogenesis
Retinoic acid	Apoptosis		Impaired	Hypoplastic	Normal	NA	EDE
Forskolin	Cyclic nucleotides	Adenylate cyclase	Impaired	Hypoplastic	Normal	NA	EDE
L-162,313	Neurotransmission	AT1	Impaired	Hypoplastic	Normal	Normal	LET
Indirubin-3′-oxime	Phosphorylation	CDK	Impaired	Normal	Normal	Normal	LET
SU 5416	Phosphorylation	VEGFR PTK	Impaired	Hypoplastic	Normal	NA	LET
Ectopic angiogenic sprouting
Calmidazolium chloride	Intracellular calcium	Ca²⁺ATPase	Ectopic ISVs	Hyperplastic	Normal	NA	LET
GW5074	Phosphorylation	Raf1 kinase	Ectopic ISVs	Dysplastic	Normal	NA	EDE
VVN hypoplasia
YC-1	Cyclic nucleotides	Guanylyl cyclase	Normal	Hypoplastic	Normal	Normal	EDE
Zardaverine	Cyclic nucleotides	PDE III/PDE IV	Normal	Hypoplastic	Normal	NA	EDE
MK-886	Leukotriene		Normal	Hypoplastic	Normal	NA	LET
Purvalanol A	Phosphorylation	CDK	Normal	Hypoplastic	Normal	Normal	LET

Phenotype class/compound name	Pharmacologic class	Selectivity	ISV phenotype	VVN phenotype	PCV phenotype	Lymphatic phenotype	Embryonic phenotype
Defective vasculogenesis
Nocodazole	Cytoskeleton and ECM	Beta-tubulin	Impaired	Hypoplastic	Hypoplastic	NA	EDE
Podophyllotoxin	Cytoskeleton and ECM		Impaired	Hypoplastic	Hypoplastic	NA	EDE
2-Methoxyestradiol	Hormone	Estrogen	Impaired	Hypoplastic	Hypoplastic	NA	EDE
IC 261	Phosphorylation	CK-1delta/epsilon	Impaired	Hypoplastic	Hypoplastic	NA	EDE
NSC 95397	Phosphorylation	Cdc25	Impaired	Hypoplastic	Hypoplastic	NA	LET
Tyrphostin AG 494	Phosphorylation	EGFR	Impaired	Hypoplastic	Hypoplastic	NA	EDE
Meclofenamic acid sodium	Prostaglandin	COX/5-lipoxygenase	Impaired	Hypoplastic	Hypoplastic	NA	EDE
Defective angiogenesis
Retinoic acid	Apoptosis		Impaired	Hypoplastic	Normal	NA	EDE
Forskolin	Cyclic nucleotides	Adenylate cyclase	Impaired	Hypoplastic	Normal	NA	EDE
L-162,313	Neurotransmission	AT1	Impaired	Hypoplastic	Normal	Normal	LET
Indirubin-3′-oxime	Phosphorylation	CDK	Impaired	Normal	Normal	Normal	LET
SU 5416	Phosphorylation	VEGFR PTK	Impaired	Hypoplastic	Normal	NA	LET
Ectopic angiogenic sprouting
Calmidazolium chloride	Intracellular calcium	Ca²⁺ATPase	Ectopic ISVs	Hyperplastic	Normal	NA	LET
GW5074	Phosphorylation	Raf1 kinase	Ectopic ISVs	Dysplastic	Normal	NA	EDE
VVN hypoplasia
YC-1	Cyclic nucleotides	Guanylyl cyclase	Normal	Hypoplastic	Normal	Normal	EDE
Zardaverine	Cyclic nucleotides	PDE III/PDE IV	Normal	Hypoplastic	Normal	NA	EDE
MK-886	Leukotriene		Normal	Hypoplastic	Normal	NA	LET
Purvalanol A	Phosphorylation	CDK	Normal	Hypoplastic	Normal	Normal	LET

ISV indicates intersomitic vessels; VVN, vitelline vein network; PCV, posterior cardinal vein; NA, not applicable; EDE, edema phenotype; ECM, extracellular matrix; LET, lethal before stage 48; ALS, anterior lymph sac; ALH, anterior lymph heart; PLV, posterior lymph vessels; Naphthyridine, 7-chloro-4-hydroxy-2-phenyl-1,8-naphthyridine; and 7-Cyclo, 7-cyclopentyl-5-(4-phenoxy)phenyl-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine.

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