Table 1 Sequences of RT-PCR primers .... | American Society of Hematology

Table 1

Sequences of RT-PCR primers

	Primers, 5′-3′		PCR cycles
	Sense	Antisense	PCR cycles
MMP1	TTCAGCTAGCTCAGGATGAC	GCAGCATCGATATGCTTCAC	30
MMP2	CCGCCTTTAACTGGAGCAAA	TTTGGTTCTCCAGCTTCAGG	30
MMP3	CAGGTGTGGAGTTCCTGATG	GAGGTCCATAGAGGGACTGA	30
MMP7	TTTGATGGGCCAGGAAACAC	ACTTGGTCCACCTGGTTCAA	30
MMP9	GAAGATGCTGCTGTTCAGCG	ACTTGGTCCACCTGGTTCAA	30
MMP10	TTCTCCAGGCTGTATGAAGG	GAAGGACAAAGCAGGATCAC	30
MMP11	CAGAGGCCCTAAAGGTATGG	GACTCAGTGGGTAGCGAAAG	30
MMP24	GGCAGAACTGGTTAAAGTCC	CTTCTGCCACACATCGAAAG	30
MMP25	CAGTGGCCACCATGCGTAAG	AGTGAGTGTCCCCGGAGATG	30
MMP28	CGGATACCTCAATGAACAGG	CGGTGTCTAGCAAACAAGTC	30
ADAM9	GGAGCTGTTCATTGTCGTAG	GTCTCCACAGTGATTTGTCC	30
ADAM10	ACCAGATGACTGGTGTAGAG	TCGGTCTGTGAAGACATAGG	30
ADAM12	CAAGACGGTACTGATGTCTC	CTACCAACACGATCCGAATG	30
ADAM15	GGACGATCTCCCAATTAGCC	CTGCTCCAGGGTATAGCTTC	30
ADAM17	ATTATTGGTGGTAGCAGATC	GAGCCAACATAAGCTAATCC	30
ADAM19	ACCTCAGCTACGTCATCGAG	ACTCCTCCAGACTGGTACAC	30
ADAM28	ACAGAACTTGCTGGAACGAC	TCTCCCATTTCCACCAACTG	30
ADAM33	ACTACCAAGGGCGAGTAAGG	TGAGAAGCTGGTCCACGTAG	30
TIMP1	AATTCCGACCTCGTCATCAG	AAGCAATGAGTGCCACTCTG	30
TIMP2	ATGCAGATGTAGTGATCAGG	CGTTGATGTTCTTCTCTGTG	30
TIMP3	GCAGATGAAGATGTACCGAG	GTCTGTGGCATTGATGATGC	30
GAPDH	TGTCTTCACCACCATGGAGAAGG	GTGGATGCAGGGATGATGTTCTG	24

	Primers, 5′-3′		PCR cycles
	Sense	Antisense	PCR cycles
MMP1	TTCAGCTAGCTCAGGATGAC	GCAGCATCGATATGCTTCAC	30
MMP2	CCGCCTTTAACTGGAGCAAA	TTTGGTTCTCCAGCTTCAGG	30
MMP3	CAGGTGTGGAGTTCCTGATG	GAGGTCCATAGAGGGACTGA	30
MMP7	TTTGATGGGCCAGGAAACAC	ACTTGGTCCACCTGGTTCAA	30
MMP9	GAAGATGCTGCTGTTCAGCG	ACTTGGTCCACCTGGTTCAA	30
MMP10	TTCTCCAGGCTGTATGAAGG	GAAGGACAAAGCAGGATCAC	30
MMP11	CAGAGGCCCTAAAGGTATGG	GACTCAGTGGGTAGCGAAAG	30
MMP24	GGCAGAACTGGTTAAAGTCC	CTTCTGCCACACATCGAAAG	30
MMP25	CAGTGGCCACCATGCGTAAG	AGTGAGTGTCCCCGGAGATG	30
MMP28	CGGATACCTCAATGAACAGG	CGGTGTCTAGCAAACAAGTC	30
ADAM9	GGAGCTGTTCATTGTCGTAG	GTCTCCACAGTGATTTGTCC	30
ADAM10	ACCAGATGACTGGTGTAGAG	TCGGTCTGTGAAGACATAGG	30
ADAM12	CAAGACGGTACTGATGTCTC	CTACCAACACGATCCGAATG	30
ADAM15	GGACGATCTCCCAATTAGCC	CTGCTCCAGGGTATAGCTTC	30
ADAM17	ATTATTGGTGGTAGCAGATC	GAGCCAACATAAGCTAATCC	30
ADAM19	ACCTCAGCTACGTCATCGAG	ACTCCTCCAGACTGGTACAC	30
ADAM28	ACAGAACTTGCTGGAACGAC	TCTCCCATTTCCACCAACTG	30
ADAM33	ACTACCAAGGGCGAGTAAGG	TGAGAAGCTGGTCCACGTAG	30
TIMP1	AATTCCGACCTCGTCATCAG	AAGCAATGAGTGCCACTCTG	30
TIMP2	ATGCAGATGTAGTGATCAGG	CGTTGATGTTCTTCTCTGTG	30
TIMP3	GCAGATGAAGATGTACCGAG	GTCTGTGGCATTGATGATGC	30
GAPDH	TGTCTTCACCACCATGGAGAAGG	GTGGATGCAGGGATGATGTTCTG	24

MMP1 indicates matrix metalloproteinase-1; ADAM, a disintegrin and metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; and GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

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