Table 2 Diagnostic PCR primers used to... | American Society of Hematology

Table 2

Diagnostic PCR primers used to identify splicing alterations in the FPGS gene (accession no. NM_004957)

Primer	Position on gDNA	Length, bp
INT10 5′-GAGTGGGCAGCTGAGTGGG-3′*	Intron 10	1368
ex9-10 5′-GCCCAGCAGATCTCATGTCC-3′*	3′ of exon 9+ 5′ of exon 10	449
ex9-11 5′-CAGATCTCATGCTGGGGAGC-3′*	3′ of exon 9+ 5′ of exon 11	294
ex10in10 5′-AGGACCGCCATGGTGAGTG-3′*	3′ of exon 10+ 5′ of intron 10	393
ex11-12 5′-GCTCGGGCTTCGGAACAC-3′†	3′ of exon 11+ 5′ of exon 12	387
ex11-13 5′-CCCACATGCGGCTCGTG-3′†	3′ of exon 11+ 5′ of exon 13	245

Primer	Position on gDNA	Length, bp
INT10 5′-GAGTGGGCAGCTGAGTGGG-3′*	Intron 10	1368
ex9-10 5′-GCCCAGCAGATCTCATGTCC-3′*	3′ of exon 9+ 5′ of exon 10	449
ex9-11 5′-CAGATCTCATGCTGGGGAGC-3′*	3′ of exon 9+ 5′ of exon 11	294
ex10in10 5′-AGGACCGCCATGGTGAGTG-3′*	3′ of exon 10+ 5′ of intron 10	393
ex11-12 5′-GCTCGGGCTTCGGAACAC-3′†	3′ of exon 11+ 5′ of exon 12	387
ex11-13 5′-CCCACATGCGGCTCGTG-3′†	3′ of exon 11+ 5′ of exon 13	245

Forward primer used with the reverse primer EX13-dw from Table 1.

Forward primer used with the reverse primer EX15-dw from Table 1.