Evidence for Preferential Association of Iron With HbS
| Experimental Condition . | Hb Oxidation Rate (μmol/L/min) . | |
|---|---|---|
| HbA . | HbS . | |
| 1. Standard Hb preparation | .028 ± .001 | .047 ± .001 |
| 2. #1 with iron removed | .029 ± .001 | .034 ± .003 |
| 3. #2 reexposed to iron | .094 ± .003 | .145 ± .012 |
| 4. Incremental rate (#3 minus #2) | .065 ± .003 | .114 ± .014* |
| 5. #3 with DTPA added | .029 ± .002 | .035 ± .003 |
| Experimental Condition . | Hb Oxidation Rate (μmol/L/min) . | |
|---|---|---|
| HbA . | HbS . | |
| 1. Standard Hb preparation | .028 ± .001 | .047 ± .001 |
| 2. #1 with iron removed | .029 ± .001 | .034 ± .003 |
| 3. #2 reexposed to iron | .094 ± .003 | .145 ± .012 |
| 4. Incremental rate (#3 minus #2) | .065 ± .003 | .114 ± .014* |
| 5. #3 with DTPA added | .029 ± .002 | .035 ± .003 |
Results are shown as the mean ± SD (n = 3 for each) and expressed as for Table 1. Experimental conditions: see text. Note that reexposure to iron (line 3) involved 50 μmol/L iron with 1 mmol/L ADP, so the resulting incremental oxidation rate (line 4) can be compared directly with the ADP experiment shown in Table 3.
(P = .004 for HbA v HbS, indicating preferential association of iron with HbS.