Release of Sialidase From Human Erythrocyte Resealed Vesicles by Exhaustive Treatment With PIPLC Followed by Centrifugation
| . | Resealed Vesicles . | Supernatant . | Pellet . |
|---|---|---|---|
| Total protein (mg) | 2.31 | 0.27 | 1.87 |
| % | 100 | 11.6 | 80.8 |
| Acidic sialidase | |||
| μU/mg protein | 14.3 ± 0.7 | 103.7 ± 5.5 | ND |
| Total μU | 33.1 | 27.8 | ND |
| % | 100 | 84 | ND |
| Neutral sialidase | |||
| μU/mg protein | 56.9 ± 2.3 | ND | 66.6 ± 4.6 |
| Total μU | 131.7 | ND | 124.5 |
| % | 100 | 0 | 94.5 |
| . | Resealed Vesicles . | Supernatant . | Pellet . |
|---|---|---|---|
| Total protein (mg) | 2.31 | 0.27 | 1.87 |
| % | 100 | 11.6 | 80.8 |
| Acidic sialidase | |||
| μU/mg protein | 14.3 ± 0.7 | 103.7 ± 5.5 | ND |
| Total μU | 33.1 | 27.8 | ND |
| % | 100 | 84 | ND |
| Neutral sialidase | |||
| μU/mg protein | 56.9 ± 2.3 | ND | 66.6 ± 4.6 |
| Total μU | 131.7 | ND | 124.5 |
| % | 100 | 0 | 94.5 |
For details, see Materials and Methods. The data are the mean values of five experiments ± SD values.
Abbreviation: ND, not detectable.