Cytotoxic Effect of Dexamethasone and Fludarabine on B-CLL Lymphocytes
| Patient . | % Viability . | ||
|---|---|---|---|
| No. . | Control . | Dexamethasone . | Fludarabine . |
| 1 | 99 ± 9 | ND | 54 ± 9 |
| 2 | 102 ± 18 | 31 ± 2 | 28 ± 2 |
| 3 | 65 ± 1.5 | 52 ± 1 | 27 ± 3 |
| 4 | 29 ± 1 | 13 ± 0.2 | 7 ± 0.1 |
| 5 | 55 ± 3 | 25 ± 2 | 21 ± 2 |
| 6 | 25 ± 1 | 18 ± 1 | 15 ± 1 |
| 7 | 83 ± 13 | 52 ± 12 | 37 ± 19 |
| 8 | 69 ± 0.3 | 22 ± 0.3 | 26 ± 2 |
| 9 | 65 ± 4 | 27 ± 0.4 | 32 ± 2 |
| 10 | 100 ± 2.5 | ND | 32 ± 4 |
| 11 | 68 ± 2 | 50 ± 0.3 | 25 ± 5 |
| Patient . | % Viability . | ||
|---|---|---|---|
| No. . | Control . | Dexamethasone . | Fludarabine . |
| 1 | 99 ± 9 | ND | 54 ± 9 |
| 2 | 102 ± 18 | 31 ± 2 | 28 ± 2 |
| 3 | 65 ± 1.5 | 52 ± 1 | 27 ± 3 |
| 4 | 29 ± 1 | 13 ± 0.2 | 7 ± 0.1 |
| 5 | 55 ± 3 | 25 ± 2 | 21 ± 2 |
| 6 | 25 ± 1 | 18 ± 1 | 15 ± 1 |
| 7 | 83 ± 13 | 52 ± 12 | 37 ± 19 |
| 8 | 69 ± 0.3 | 22 ± 0.3 | 26 ± 2 |
| 9 | 65 ± 4 | 27 ± 0.4 | 32 ± 2 |
| 10 | 100 ± 2.5 | ND | 32 ± 4 |
| 11 | 68 ± 2 | 50 ± 0.3 | 25 ± 5 |
B-CLL lymphocytes were incubated at 37°C for 48 hours in the absence of any factor (control) or with 10 μmol/L dexamethasone or fludarabine (5 μg/mL). Cell viability was determined by the MTT assay as described in Materials and Methods. Data are shown as the mean value ± SD.
Abbreviation: ND, not determined.