Expansion of mHA-specific Tregs
| . | . | mHA-specific Treg FPM* . | . | |
|---|---|---|---|---|
| Sibling pair . | Samples from blood/apheresis . | Before culture . | After culture . | mHA-specific Treg fold expansion . |
| 1 | Blood | 28 | 18 182 | 405 |
| 2 | Blood | 50 | 11 111 | 167 |
| 3 | Blood | 91 | 14 925 | 155 |
| 4 | Blood | 30 | 14 225 | 358 |
| Median | Blood | 40 | 14 575 | 263 |
| Range | (28-91) | (11 111-18 182) | (155-405) | |
| 5 | Apheresis | 70 | 27 248 | 324 |
| 6 | Apheresis | 19 | 8475 | 405 |
| 7 | Apheresis | 46 | 17 762 | 336 |
| 8 | Apheresis | 74 | 15 765 | 212 |
| Median | Apheresis | 58 | 16 764 | 330 |
| Range | (19-74) | (8475-27 248) | (212-405) | |
| . | . | mHA-specific Treg FPM* . | . | |
|---|---|---|---|---|
| Sibling pair . | Samples from blood/apheresis . | Before culture . | After culture . | mHA-specific Treg fold expansion . |
| 1 | Blood | 28 | 18 182 | 405 |
| 2 | Blood | 50 | 11 111 | 167 |
| 3 | Blood | 91 | 14 925 | 155 |
| 4 | Blood | 30 | 14 225 | 358 |
| Median | Blood | 40 | 14 575 | 263 |
| Range | (28-91) | (11 111-18 182) | (155-405) | |
| 5 | Apheresis | 70 | 27 248 | 324 |
| 6 | Apheresis | 19 | 8475 | 405 |
| 7 | Apheresis | 46 | 17 762 | 336 |
| 8 | Apheresis | 74 | 15 765 | 212 |
| Median | Apheresis | 58 | 16 764 | 330 |
| Range | (19-74) | (8475-27 248) | (212-405) | |
FPM, frequency per million.
mHA-specific Treg frequencies were calculated by LDA of purified Tregs before and 12 days after culture.