ISG expression in HSV-2–treated MDDCs
| Symbol . | Accession no. . | Microarray (6 hours) . | qPCR (6 hours) . |
|---|---|---|---|
| IFNB1 | NM_002176 | 14.34/14.39 | 600 |
| OAS1* | NM_016816 | 8.06/1.04 | 18 |
| OAS2 | NM_016817 | 7.96/0.20 | — |
| OAS3 | NM_006187 | 9.25/2.94 | — |
| MX1* | NM_002462 | 6.40/−4.06 | 105 |
| MX2 | NM_002463 | 3.88/1.03 | — |
| PKR* | NM_002759 | 6.11/3.7 | 20 |
| IRF-1* | NM_002198 | 5.45/0.82 | 10.3 |
| IRF-9* | NM_006084 | 2.46/1.62 | 5.5 |
| GBP1 | NM_002053 | 11.90/3.46 | — |
| HERC5 | NM_016323 | 22.74/4.87 | — |
| IFI16 | NM_005531 | 2.27/6.22 | — |
| IFI35* | NM_005533 | 4.93/2.18 | — |
| IFI44 | NM_006417 | 11.99/3.99 | — |
| IFI44L | NM_006820 | 24.35/4.17 | — |
| IFIH1 | NM_022168 | 7.51/4.14 | — |
| IFIT1* | NM_001548 | 30.47/2.94 | 30 |
| IFIT2* | NM_001547 | 30.09/2.26 | 9 |
| IFIT3* | NM_001549 | 38.83/5.78 | — |
| IFIT5* | NM_012420 | 2.38/5.03 | — |
| IFITM1* | NM_003641 | 14.27/2.54 | — |
| IFITM2 | NM_006435 | 5.67/3.41 | — |
| IFITM3* | NM_021034 | 6.75/3.65 | — |
| IL27 | NM_145659 | 4.28/1.59 | — |
| ISG15* | NM_005101 | 13.72/2.40 | — |
| ISG20 | NM_002201 | 33.32/6.48 | — |
| JAK2 | NM_004972 | 2.40/0.82 | — |
| LOC400759 | XR_000992 | 6.05/3.19 | — |
| PSMB9* | NM_002800 | 2.25/4.46 | — |
| SP110* | NM_080424 | 2.40/2.45 | — |
| STAT1* | NM_139266 | 7.34/2.41 | — |
| UBA7 | NM_003335 | 2.52/2.09 | — |
| DDX58* | NM_014314 | 11.78/3.54 | — |
| DDX60 | NM_017631 | 5.29/3.45 | — |
| DHX58 | NM_017631 | 4.44/0.65 | — |
| WARS* | NM_004184 | 2.71/1.00 | — |
| Symbol . | Accession no. . | Microarray (6 hours) . | qPCR (6 hours) . |
|---|---|---|---|
| IFNB1 | NM_002176 | 14.34/14.39 | 600 |
| OAS1* | NM_016816 | 8.06/1.04 | 18 |
| OAS2 | NM_016817 | 7.96/0.20 | — |
| OAS3 | NM_006187 | 9.25/2.94 | — |
| MX1* | NM_002462 | 6.40/−4.06 | 105 |
| MX2 | NM_002463 | 3.88/1.03 | — |
| PKR* | NM_002759 | 6.11/3.7 | 20 |
| IRF-1* | NM_002198 | 5.45/0.82 | 10.3 |
| IRF-9* | NM_006084 | 2.46/1.62 | 5.5 |
| GBP1 | NM_002053 | 11.90/3.46 | — |
| HERC5 | NM_016323 | 22.74/4.87 | — |
| IFI16 | NM_005531 | 2.27/6.22 | — |
| IFI35* | NM_005533 | 4.93/2.18 | — |
| IFI44 | NM_006417 | 11.99/3.99 | — |
| IFI44L | NM_006820 | 24.35/4.17 | — |
| IFIH1 | NM_022168 | 7.51/4.14 | — |
| IFIT1* | NM_001548 | 30.47/2.94 | 30 |
| IFIT2* | NM_001547 | 30.09/2.26 | 9 |
| IFIT3* | NM_001549 | 38.83/5.78 | — |
| IFIT5* | NM_012420 | 2.38/5.03 | — |
| IFITM1* | NM_003641 | 14.27/2.54 | — |
| IFITM2 | NM_006435 | 5.67/3.41 | — |
| IFITM3* | NM_021034 | 6.75/3.65 | — |
| IL27 | NM_145659 | 4.28/1.59 | — |
| ISG15* | NM_005101 | 13.72/2.40 | — |
| ISG20 | NM_002201 | 33.32/6.48 | — |
| JAK2 | NM_004972 | 2.40/0.82 | — |
| LOC400759 | XR_000992 | 6.05/3.19 | — |
| PSMB9* | NM_002800 | 2.25/4.46 | — |
| SP110* | NM_080424 | 2.40/2.45 | — |
| STAT1* | NM_139266 | 7.34/2.41 | — |
| UBA7 | NM_003335 | 2.52/2.09 | — |
| DDX58* | NM_014314 | 11.78/3.54 | — |
| DDX60 | NM_017631 | 5.29/3.45 | — |
| DHX58 | NM_017631 | 4.44/0.65 | — |
| WARS* | NM_004184 | 2.71/1.00 | — |
Day 6 MDDCs were treated with HSV-2186 (MOI, 3) for 30 minutes or 6 hours, and ISG expression was determined by 48K Illumina microarrays or qPCR (6 hours only). Expression data from 3 independent experiments are presented for HSV-2– versus mock-treated cells. In cells that were treated for 30 minutes, the ISG expression was determined by qPCR. Data are fold change ± B value.
The — indicates that qPCR was performed to confirm the expression of this gene.
Genes were also detected to be increased in their expression in MDDCs exposed to HIV-1BaL (Table 1).