Inhibition of shedding by the ADAM17-specific inhibitor, BMS-561392
| Protein name . | Accession no. . | No. of transmembrane domains . | Percentage inhibition . |
|---|---|---|---|
| Junctional adhesion molecule (JAM-A) | Q9Y5B2 | 1 | 100 |
| Ephrin B1 | P98172 | 1 | 100 |
| Receptor-type tyrosine phosphatase G | P23470 | 1 | 100 |
| Soluble calcium-activated nucleotidase (SCAN-1) | Q8WVQ1 | 1 | 100 |
| Platelet glycoprotein IX (GPIX) | P14770 | 1 | 100 |
| Tetraspanin 9 | O75954 | 4 | 100 |
| Semaphorin 7A | O75326 | 0 (GPI) | 100 |
| Semaphorin 4D | Q92854 | 1 | 85 |
| Fibrocystin-L | Q86WI1 | 1 | 84 |
| Platelet glycoprotein V (GPV) | P40197 | 1 | 64 |
| Platelet glycoprotein Ibα chain (GPIbα) | P07359 | 1 | 63 |
| D-glucuronyl C5 epimerase | O94923 | 1 | 55 |
| Trem-like transcript 1 (TLT-1) | Q86YW5 | 1 | 51 |
| Syndecan 4 | P31431 | 1 | 49 |
| CD226 | Q15762 | 1 | 45 |
| SLAM family member 5 (CD84) | QUIB8 | 1 | 44 |
| Major prion protein | P04156 | 0 (GPI) | 40 |
| Plexin domain-containing protein 2 (Plxdc 2) | Q6UX71 | 1 | 33 |
| Chloride intracellular channel protein 4 (CLIC4) | Q9Y696 | 1 | 32 |
| Integrin αIIb | Q5SRI9 | 1 | 26 |
| Integrin β3 | P05106 | 1 | 20 |
| CD40-L | P29965 | 1 | 17 |
| CD9 | P21926 | 4 | 17 |
| Dystroglycan | Q14118 | 1 | 11 |
| Platelet glycoprotein VI (GPVI) | Q9HCN6 | 1 | 2 |
| Amyloid βA4 | P05067 | 1 | 0 |
| P-selectin | P16109 | 1 | 0 |
| Amyloid-like protein 2 | Q06481 | 1 | 0 |
| Chloride intracellular channel protein 1 (CLIC1) | O00299 | 1 | 0 |
| Endothelial cell selective adhesion molecule (ESAM) | Q96AP7 | 1 | 0 |
| Lysosome-associated membrane glycoprotein 2 (LAMP2) | P13473 | 1 | 0 |
| Lysosomal acid phosphatase | P11117 | 1 | 0 |
| Lysosome associated membrane glycoprotein 1 (LAMP1) | P11279 | 1 | 0 |
| Trem-like transcript 2 (TLT-2) | Q5T2D2 | 1 | 0 |
| Inositol monophosphatase 3 | Q9NX62 | 1 | 0 |
| Receptor-type tyrosine phosphatase γ (CD148) | Q12913 | 1 | 0 |
| VCAM1 | P19320 | 1 | 0 |
| Endothelial cell scavenger receptor | Q14162 | 1 | 0 |
| IL6 receptor | P40189 | 1 | 0 |
| CD109 | Q6YHK3 | 0 (GPI) | 0 |
| Protein name . | Accession no. . | No. of transmembrane domains . | Percentage inhibition . |
|---|---|---|---|
| Junctional adhesion molecule (JAM-A) | Q9Y5B2 | 1 | 100 |
| Ephrin B1 | P98172 | 1 | 100 |
| Receptor-type tyrosine phosphatase G | P23470 | 1 | 100 |
| Soluble calcium-activated nucleotidase (SCAN-1) | Q8WVQ1 | 1 | 100 |
| Platelet glycoprotein IX (GPIX) | P14770 | 1 | 100 |
| Tetraspanin 9 | O75954 | 4 | 100 |
| Semaphorin 7A | O75326 | 0 (GPI) | 100 |
| Semaphorin 4D | Q92854 | 1 | 85 |
| Fibrocystin-L | Q86WI1 | 1 | 84 |
| Platelet glycoprotein V (GPV) | P40197 | 1 | 64 |
| Platelet glycoprotein Ibα chain (GPIbα) | P07359 | 1 | 63 |
| D-glucuronyl C5 epimerase | O94923 | 1 | 55 |
| Trem-like transcript 1 (TLT-1) | Q86YW5 | 1 | 51 |
| Syndecan 4 | P31431 | 1 | 49 |
| CD226 | Q15762 | 1 | 45 |
| SLAM family member 5 (CD84) | QUIB8 | 1 | 44 |
| Major prion protein | P04156 | 0 (GPI) | 40 |
| Plexin domain-containing protein 2 (Plxdc 2) | Q6UX71 | 1 | 33 |
| Chloride intracellular channel protein 4 (CLIC4) | Q9Y696 | 1 | 32 |
| Integrin αIIb | Q5SRI9 | 1 | 26 |
| Integrin β3 | P05106 | 1 | 20 |
| CD40-L | P29965 | 1 | 17 |
| CD9 | P21926 | 4 | 17 |
| Dystroglycan | Q14118 | 1 | 11 |
| Platelet glycoprotein VI (GPVI) | Q9HCN6 | 1 | 2 |
| Amyloid βA4 | P05067 | 1 | 0 |
| P-selectin | P16109 | 1 | 0 |
| Amyloid-like protein 2 | Q06481 | 1 | 0 |
| Chloride intracellular channel protein 1 (CLIC1) | O00299 | 1 | 0 |
| Endothelial cell selective adhesion molecule (ESAM) | Q96AP7 | 1 | 0 |
| Lysosome-associated membrane glycoprotein 2 (LAMP2) | P13473 | 1 | 0 |
| Lysosomal acid phosphatase | P11117 | 1 | 0 |
| Lysosome associated membrane glycoprotein 1 (LAMP1) | P11279 | 1 | 0 |
| Trem-like transcript 2 (TLT-2) | Q5T2D2 | 1 | 0 |
| Inositol monophosphatase 3 | Q9NX62 | 1 | 0 |
| Receptor-type tyrosine phosphatase γ (CD148) | Q12913 | 1 | 0 |
| VCAM1 | P19320 | 1 | 0 |
| Endothelial cell scavenger receptor | Q14162 | 1 | 0 |
| IL6 receptor | P40189 | 1 | 0 |
| CD109 | Q6YHK3 | 0 (GPI) | 0 |
Percentage inhibition by BMS-561392 is expressed as: 1 − the ratio of spectral counts in the presence and absence of the inhibitor.