Patient and donor characteristics
| . | Patient 1 . | Patient 2 . |
|---|---|---|
| Diagnosis | AML-M1 | AML-M0 |
| Age, y | 66 | 58 |
| Gender of patient/donor | M/M | M/F |
| CMV serostatus of patient/donor | +/+ | +/+ |
| EBV serostatus of patient/donor | +/– | +/+ |
| HLA-DP typing* | ||
| Patient | B1*0101/B1*0301 A1*0103/A1*0201 | B1*0301/B1*0401 A1*0103 |
| Donor | B1*0201/B1*0401 A1*0103 | B1*0201/B1*0401 A1*0103 |
| T-cell composition of CD4+ DLI | ||
| CD3+CD4+ T cells, % | 98.29 | 98.22 |
| CD3+CD8+ T cells, % | 1.23 | 1.46 |
| CD3+CD4+CD8+ T cells, % | 0.11 | 0.22 |
| CD3+CD4-CD8- T cells, % | 0.36 | 0.22 |
| . | Patient 1 . | Patient 2 . |
|---|---|---|
| Diagnosis | AML-M1 | AML-M0 |
| Age, y | 66 | 58 |
| Gender of patient/donor | M/M | M/F |
| CMV serostatus of patient/donor | +/+ | +/+ |
| EBV serostatus of patient/donor | +/– | +/+ |
| HLA-DP typing* | ||
| Patient | B1*0101/B1*0301 A1*0103/A1*0201 | B1*0301/B1*0401 A1*0103 |
| Donor | B1*0201/B1*0401 A1*0103 | B1*0201/B1*0401 A1*0103 |
| T-cell composition of CD4+ DLI | ||
| CD3+CD4+ T cells, % | 98.29 | 98.22 |
| CD3+CD8+ T cells, % | 1.23 | 1.46 |
| CD3+CD4+CD8+ T cells, % | 0.11 | 0.22 |
| CD3+CD4-CD8- T cells, % | 0.36 | 0.22 |
CMV and EBV serostatus was determined by standard serology methods: +, positive; –, negative.
Mismatched patient HLA-DPB1 and HLA-DPA1 alleles are underlined.
HLA class I and HLA class II typing was performed by DNA analysis using sequence-specific primers. Patients and their URD were fully matched at HLA-A, -B, -C, -DRB, and -DQB1 alleles (not shown).